F8666
Lipopolysaccharides from Escherichia coli O55:B5
FITC conjugate
동의어(들):
LPS
생물학적 소스
Escherichia coli (O55:B5)
Quality Level
결합
FITC conjugate
양식
lyophilized powder
라벨링 범위
2-10 μg FITC per mg LPS
색상
yellow to orange
solubility
H2O: 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow
배송 상태
ambient
저장 온도
2-8°C
일반 설명
This product is a TCA extract of E. coli serotype O55:B5 (strain CDC 1644-70), which was purified by gel filtration and conjugated to FITC (fluorescein isothiocyanate).
애플리케이션
FITC-Lipopolysaccharides (FITC-LPS) may be used to study the movement/transport of LPS across tissue barriers such as the colonic epithelium and cellular binding and internalization of lipopolysaccharides with fluorescence-based assay systems. FITC-LPS may be used to evaluate, qualify and quantify lipopolysaccharides (LPS) removal reagents and procedures. FITC-LPS conjugate may be used for the detection of LPA binding proteins and motifs.
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).
생화학적/생리학적 작용
Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.
제조 메모
The product is soluble in water (5 mg/ml), yielding a hazy faint yellow solution. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.
기타 정보
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.
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신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 2 Oral
Storage Class Code
6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
WGK
WGK 3
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
개인 보호 장비
Eyeshields, Gloves, type N95 (US)