G0535

Glycopeptidase A from almonds

buffered aqueous glycerol solution, ≥0.05 unit/mL

• 동의어(들): N-Glycosidase A, N-linked-glycopeptide-(N-acetyl-β-D-glucosaminyl)-L-asparagine amidohydrolase, PNGase A
• CAS Number: 83534-39-8
• EC 번호: 3.5.1.52 (BRENDA, IUBMB)
• MDL number: MFCD00131222
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 결합: (N-linked)
• Quality Level: 200
• 양식: buffered aqueous glycerol solution
• 농도: ≥0.05 unit/mL
• 저장 온도: −20°C

설명

일반 설명

Glycopeptidase found in almonds can be divided into three groups- A, B and C. the optimum pH value and the isoelectric point of glycopeptidase A is 6.0 and 7.7 respectively. It has a preference for glycopeptides with long chains. It is also capable of hydrolyzing intact glycoproteins such as, desialyted human transferrin, ovalbumin etc. These proteins cleave glycoproteins with asialocarbohydrate moieties at their β-aspartyl-glucosylamine linkages.

애플리케이션

Glycopeptidase A from almonds is used for deglycosylation. It catalyzes the removal of N-linked oligosaccharide chains and converts Asn residue to Asp.

생화학적/생리학적 작용

Hydrolyzes an N⁴-(acetyl-β-D-glycosaminyl)asparagine in which the N-acetyl-D-glucosamine residue may be further glycosylated, yielding a (substituted) N-acetyl-β-D-glucoaminylamine and the peptide containing an aspartic residue.

단위 정의

One unit will hydrolyze 1.0 μmole of ovalbumin glycopeptide per min at pH 5.0 at 37°C.

물리적 형태

Solution in 50% glycerol containing 50 mM citrate-phosphate buffer, pH 5.0, and BSA.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): No data available
• Flash Point (°C): No data available