G1924

L-Glutamate Oxidase from Streptomyces sp.

recombinant, expressed in E. coli

• 동의어(들): L-Glutamate:oxygen oxidoreductase (deaminating)
• CAS Number: 39346-34-4
• MDL number: MFCD00131459
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in E. coli
• Quality Level: 200
• 양식: solid
• 특이 활성도: ≥5 units/mg protein
• 분자량: 120 kDa
• 저장 온도: −20°C

설명

일반 설명

L-Glutamate Oxidase (LGOX) from Streptomyces sp is secreted extracellularly in the wheat bran pre cultivation medium. It corresponds to a molecular weight of 120 kDa with monomer subunit of 61 kDa. The structure of LGOX comprises of hexamer and has flavin adenine dinucleotide prosthetic group.

This product has a 6xHistidine tag with a thrombin cleavage site.

애플리케이션

L-Glutamate Oxidase from Streptomyces sp may be used:

• as a reference enzyme in hydrogelpoly(carbamoylsulphonate) based immobilization for biosensor development
• as a component of pencil graphite electrode for biosensing L-glutamate

생화학적/생리학적 작용

L-Glutamate Oxidase (LGOX) catalyzes the oxidative deamination of L-glutamate to α-ketoglutarate, ammonia and hydrogen peroxide. LGOX is industrially produced and is exploited majorly as a biosensor for L-glutamate detection and in the L-glutamate kits.

단위 정의

One unit will form 1.0 micromole of alpha-ketoglutaric acid from L-glutamic acid per minute at pH 7.4 at 30 deg C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable