G7907

Galactose Oxidase from Dactylium dendroides

≥30 units/mg solid

• 동의어(들): D-Galactose:oxygen 6-oxidoreductase
• CAS Number: 9028-79-9
• EC 번호: 1.1.3.9 (BRENDA, IUBMB)
• EC Number: 232-843-7
• MDL number: MFCD00131118
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: fungus (Dactylium dendroides)
• Quality Level: 200
• 양식: lyophilized
• 특이 활성도: ≥30 units/mg solid
• 저장 온도: −20°C

설명

일반 설명

Galactose oxidase is a member of radicalcoupled copper oxidases family. It is a fungal secretory enzyme.

Galactose oxidase is an extracellular copper-containing enzyme, secreted by the deuteromycete fungus Dactylium dendroides. It catalyzes the oxidation of a range of primary alcohols, including D-galactose, to the corresponding aldehyde, with reduction of oxygen to hydrogen peroxide.

애플리케이션

Galactose Oxidase from Dactylium dendroides has been used as a component for galactose oxidase treatment of arabinogalactan. It has also been used to co-immobilise with peroxidase for the preparation of a biosensor for galactose detection.

Galactose oxidase may be used as an analytical tool for the specific determination of D-galactose in blood plasma, plant extracts, and phospholipids. It could be used for the characterization of terminal D-galactoside units in several polymers. It may also be useful in the determination of lactose.

생화학적/생리학적 작용

Galactose oxidase catalyzes the coversion of D-galactose to D-galacto-hexodialdose.
2-Deoxy-D-galactose, lactose, melibiose, raffinose and stachyose react with galactose oxidase in the peroxidase:o-tolidine system.
Essentially no oxidation of D-glucose, L-galactose, L-arabinose or D-glucuronate has been observed.

Galactose oxidase has several applications in bioanalytics and histology. This free radical enzyme possess high substrate specificity.

단위 정의

One unit will produce a ΔA₄₂₅ of 1.0 per min at pH 6.0 at 25 °C, in a peroxidase and o-tolidine system. Reaction volume = 3.4 mL. Light path = 1 cm.

물리적 형태

Lyophilized, contains buffer salts and stabilizer

제조 메모

Chromatographically purified

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable