G8160

Granulocyte Colony-Stimulating Factor from mouse

>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 동의어(들): G-CSF
• MDL number: MFCD00166475
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• Product Name: Granulocyte Colony-Stimulating Factor from mouse, G-CSF, recombinant, expressed in E. coli, suitable for cell culture
• 생물학적 소스: mouse
• Quality Level: 100
• 재조합: expressed in E. coli
• 분석: >97% (SDS-PAGE)
• 양식: lyophilized powder
• 효능: 10-60 pg/mL ED₅₀
• 품질: endotoxin tested
• 분자량: 19 kDa (179 amino acids including N-terminal methionine)
• 포장: pkg of 5 and 25 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: ≤0.01 EU/μg
• UniProt 수납 번호: P09920
• 저장 온도: −20°C
• 유전자 정보: mouse ... Csf3(12985), Csf3r(12986)

설명

일반 설명

The Csf3r (colony stimulating factor 3 receptor (granulocyte)) gene encodes a prototypical mobilizing cytokine.

애플리케이션

Granulocyte Colony-Stimulating Factor from mouse has been used in cytokine therapy.

생화학적/생리학적 작용

G-CSF is a proliferation, differentiation, survival, and activation factor for hematopoietic cells of the restricted neutrophilic granulocyte lineage. It is produced by macrophages activated by endotoxin (LPS), by monocytes activated by TNF-α with IFNγ, by fibroblasts and endothelial cells activated by IL-1 or TNF-α, and by bone marrow stromal cells activated by IL-1 or LPS. It is suggested that during the inflammatory process endotoxins stimulate tissue macrophages to produce not only G-CSF but several other cytokines, including IL-1 and TNF-α, which in turn stimulate more G-CSF release from endothelial cells and fibroblasts. G-CSF can also synergize with IL-3 to shorten the G₀ period of early hematopoietic progenitors. In addition to the namesake proliferative activity, G-CSF acts on mature neutrophils to enhance their survival and to stimulate their tumorcidal activity. Human G-CSF binds and activates a 130 kD to150 kD glycoprotein single chain receptor that has been classified as a member of the hematopoietic (cytokine) receptor family, the cytokine receptor class I, or the gp130 related cytokine receptor family (although it does not apparently bind to gp130). G-CSF receptors can be found on neutrophils, myeloid leukemia cells that respond to G-CSF, bone marrow cells of neutrophilic granulocyte lineage, and on placental trophoblasts, plus a soluble form may be expressed. Two forms of human G-CSF (177 and 174 amino acids) are synthesized from a single gene by alternative splicing, but murine G-CSF is a single expressed form of 178 amino acids. Human and murine G-CSF share 73% amino acid sequence homology and full cross-reactivity.

Granulocyte colony-stimulating factor (G-CSF) is a lineage-restricted hematopoietic growth factor, stimulating final mitotic divisions and the terminal cellular maturation of the partially differentiated hematopoietic progenitors. G-CSF^3,4 is produced by monocytes and fibroblasts. It stimulates granulocyte colony formation, activates neutrophils and mature granulocytes, and promotes differentiation of certain myeloid leukemic cells. Natural G-CSF is a glycoprotein of 177 amino acids and a molecular weight of 19 kDa.⁵ Human and murine G-CSF have about 75% homology and show biological cross-reactivity.

물리적 형태

Lyophilized from a 0.2 μm filtered solution in 10 mM citric acid, 0.01% TWEEN^® 20, pH 4.0, containing 50 μg bovine serum albumin per 1 μg of cytokine.

분석 메모

The biological activity is measured in a cell proliferation assay using a murine myeloblastic cell line, NFS-60.

법적 정보

TWEEN is a registered trademark of Croda International PLC

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H319,H335
• 예방조치 성명서: P261 - P264 - P271 - P280 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)