G8880

Glutaminase from Escherichia coli

Grade V, lyophilized powder, 50-200 units/mg protein

• 동의어(들): L-Glutamine amidohydrolase
• CAS Number: 9001-47-2
• EC 번호: 3.5.1.2 (BRENDA, IUBMB)
• MDL number: MFCD00131193
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: Escherichia coli
• Quality Level: 200
• 유형: Grade V
• 양식: lyophilized powder
• 특이 활성도: 50-200 units/mg protein
• 분자량: 110 kDa
• 구성: Protein, ~30% biuret
• 양이온 미량물: NH₄⁺: <0.1 μg/unit
• 외래 활성: NADH Oxidase ≤0.01%
• 저장 온도: −20°C

설명

일반 설명

Glutaminase is an amidohydrolase. It has the catalytic domain in the N-terminal and the C-terminal extension possesses STAS domain.

애플리케이션

Glutaminase from E.coli has been used:

• for the determination of glutamine from the cerebral cortex tissue extract
• for immobilization on aminopropylsilylated controlled pore glass (CPG) for determination of glutamine
• in combination with acivicin in in vitro melanoma cells and MCF-7 and OAW-42 cells for cell proliferation and invasiveness assay.

생화학적/생리학적 작용

Glutaminase catalyzes the conversion of glutamine to glutamate.

Glutaminase from Escherichia coli along with acivicin, a glutamine analog regulates proliferation and invasiveness of cancer cells. Bacterial glutaminase is implicated in increasing the life span of cancer patients by lowering the tumor burden.

단위 정의

One unit will deaminate 1.0 μmole of L-glutamine per min at pH 4.9 at 37 °C.

물리적 형태

Lyophilized powder containing potassium succinate and EDTA

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)