H5505

Histone from calf thymus

Type III-S

• CAS Number: 9064-47-5
• Beilstein: 1895465
• MDL number: MFCD00131329
• UNSPSC 코드: 41106305
• NACRES: NA.51

속성

• 생물학적 소스: bovine (calf) thymus
• Quality Level: 200
• 유형: Type III-S
• 양식: powder
• 농도: ≥60% protein (biuret)
• 기술: activity assay: suitable
• solubility: H₂O: soluble 10 mg/mL, clear to hazy, colorless to light yellow
• 저장 온도: 2-8°C

설명

애플리케이션

Histone is suitable for use:

• as a positive control in the in vitro kinase assay to examine the ability of Cdc2-cyclin B1 to phosphorylate GST-CD-4 protein
• in Cdk5 activity assay
• in histone H1 kinase assay
• as a Poly(ADP-ribose) (PAR) acceptor in the assessment of in vitro PAR polymerase activity
• as an acceptor of ³²P from [γ-³²P]ATP in order to measure protein kinase activity of lysate from small and large luteal cells

생화학적/생리학적 작용

Histones are a group of proteins characterized by high levels of lysine and arginine that form reversible complexes with DNA. Based on the fractions, the molar mass of histones varies between 11-21kDa. Five different fractions have been isolated and characterized. They include H1 (lysine rich), H2a and H2b that are slightly lysine rich, H3 and H4 that are arginine rich. H1 acts as a link between "beads" (nu bodies) on the chromatin chain.

특징 및 장점

Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.

기타 정보

Characterized as mainly subgroup f₁ by SDS-PAGE

제조 메모

Lysine-rich fraction as isolated and described by de Nooij, E. and Westenbrink, H.G., Biochim. Biophys. Acta, 62, 608 (1962).

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)