H6536

Monoclonal Anti-HIF-1α antibody produced in mouse

~1 mg/mL, clone H1α67, purified immunoglobulin, buffered aqueous solution

• 동의어(들): Anti-Hypoxia Inducible Factor 1, α subunit
• MDL number: MFCD01633488
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: H1α67, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen 120 kDa
• 종 반응성: rat, sheep, ferret, human, mouse
• 농도: ~1 mg/mL
• 기술: immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:500-1:1,000 using antigen retrieval; immunoprecipitation (IP): suitable; western blot: 1:500-1:1,000
• 동형: IgG2b
• UniProt 수납 번호: Q16665
• 배송 상태: wet ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... HIF1A(3091) ; mouse ... Hif1a(15251) ; rat ... Hif1a(29560)

설명

일반 설명

Hypoxia-inducible factor-1 (HIF-1) is a 120kD basic helix-loop-helix protein that belongs to the PAS superfamily of transcription factors. It has a crucial role in activating transcription of hypoxia-inducible genes. It can also activate the expression of the gene coding for NIP3; which subsequently induces cell apoptosis under persistent oxygen deprivation. Monoclonal anti-HIF-1α antibody can be used in immunostaining to detect HIF-1α localization. It can also be used in western blotting. Mouse anti-HIF-1α antibody reacts specifically with HIF1 α subunit of sheep, mouse, rat, ferret and human.

면역원

peptide corresponding to amino acids 432-528 of the human HIF-1α protein.

애플리케이션

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Monoclonal anti-HIF-1α antibody (diluted 1: 500) can be used in immunofluorescence confocal microscopy. It can also be used in in immunoblotting, immunoprecipitation and immunohistochemistry.

물리적 형태

Solution in 0.02 M phosphate buffer containing 0.25 M sodium chloride and 0.1% sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable