I4144

IL-3 mouse

≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 동의어(들): IL-3, mIL-3, Interleukin-3 from mouse
• MDL number: MFCD00164817
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• Product Name: Interleukin-3 from mouse, IL-3, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• 생물학적 소스: mouse
• Quality Level: 200
• 재조합: expressed in E. coli
• 분석: ≥98% (SDS-PAGE)
• 양식: lyophilized powder
• 효능: 0.01-0.1 ng/mL ED₅₀/EC₅₀
• 품질: endotoxin tested
• 분자량: 15.1 kDa
• 포장: pkg of 10 and 50 μg
• 기술: cell culture | mammalian: suitable
• 불순물: ≤1.000 EU/μg
• 색상: white to yellow
• UniProt 수납 번호: P01586
• 저장 온도: −20°C
• 유전자 정보: mouse ... Il3(16187)

설명

일반 설명

Interleukin-3 is a hematopoietic growth factor, which stimulates basophil development and triggers histamine, IL-4 and IL-6 release. It improves monocyte and macrophage activation and phagocytosis, enhances the secretory function of eosinophils. Interleukin-3 promotes endothelial cell proliferation and migration.

애플리케이션

Interleukin-3 from mouse has been used for the determination of 5-lipoxygenase product leukotriene C4 (LTC4) formation. It has also been used as a component of RPMI 1640 medium for cell transfection.

생화학적/생리학적 작용

Interleukin-3 (IL-3) is a multifunctional protein, originally called colony forming unit-stimulating activity (CFU-SA), and is produced by activated T lymphocytes. IL-3 supports the formation of multilineage colonies in the early development of multipotent hematopoietic progenitor cells. IL-3 has been shown to induce colony formation of macrophages, neutrophils, mast cells, and megakaryocytes from agar-suspended bone marrow cells. IL-3 also interacts with IL-2 to stimulate growth of T lymphocytes and to induce IgG secretion from activated B cells. Other synonyms for IL-3 include pan-specific hemopoietin, multicolony stimulating factor, mast cell growth factor, burst promoting activity, histamine-producing cell stimulating factor, P cell stimulating factor, and WEHI-3 factor.

물리적 형태

Lyophilized with 0.5 mg of bovine serum albumin. Sterile filtered through a 0.2 μm filter.

분석 메모

The biological activity is measured by the dose-dependent stimulation of the proliferation of mouse M-NFS-60 cells.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)