I5904

Iberiotoxin, recombinant from Mesobuthus tamulus

≥98% (HPLC), recombinant, expressed in E. coli

• 동의어(들): IbTX
• 실험식(Hill 표기법): C₁₇₉H₂₇₆N₅₀O₅₆S₇
• Molecular Weight: 4248.86
• UNSPSC 코드: 12352200
• PubChem Substance ID: 24896080
• NACRES: NA.77

속성

• 재조합: expressed in E. coli
• Quality Level: 100
• 분석: ≥98% (HPLC)
• 저장 온도: −20°C

설명

Amino Acid Sequence

Glp-Phe-Thr-Asp-Val-Asp-Cys-Ser-Val-Ser-Lys-Glu-Cys-Trp-Ser-Val-Cys-Lys-Asp-Leu-Phe-Gly-Val-Asp-Arg-Gly-Lys-Cys-Met-Gly-Lys-Lys-Cys-Arg-Cys-Tyr-Gln [Disulfide Bridges: 7−28; 13−33; 17−35]

애플리케이션

Iberiotoxin, recombinant from Mesobuthus tamulus has been used as an inhibitor of large-conductance calcium-activated K⁺ channels to study its effect on the NLRP3 (nucleotide-binding oligomerization domain-like receptor containing pyrin domain 3) inflammasome activation in bone marrow-derived macrophages (BMDMs). It has also been used to block Ca²⁺- activated K⁺ channels to determine interleukin(IL)-1β in BMDCs following inflammasome activation.

생화학적/생리학적 작용

Exhibits the same activity as the natural form of the toxin

The single chain peptide iberiotoxin (IbTX) is a selective and reversible inhibitor of high-conductance calcium-activated potassium channels (P_K,Ca). It occurs naturally in the venom of the scorpion Buthus tamulus. IbTX also modulates the binding of charybdotoxin (CbTX) to smooth muscle sarcolemmal membranes in a non-competitive manner. IbTX is similar in size to CbTX and shares considerable sequence homology with CbTX. However, IbTX differs in its overall charge in having one fewer basic and four more acidic residues than CbTX.

특징 및 장점

This compound is featured on the Potassium Channels page of the Handbook of Receptor Classification and Signal Transduction. To browse other handbook pages, click here.

제조 메모

Soluble in aqueous buffers. Stock solutions of 1 μM can be aliquoted and stored at -20 °C for up to 3 months.

안전성 정보

• 픽토그램: GHS06
• 신호어: Danger
• 유해 및 위험 성명서: H300 + H310 + H330
• 예방조치 성명서: P260 - P262 - P264 - P280 - P302 + P352 + P310 - P304 + P340 + P310
• Hazard Classifications: Acute Tox. 1 Inhalation - Acute Tox. 2 Dermal - Acute Tox. 2 Oral
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• 개인 보호 장비: Eyeshields, Faceshields, Gloves, type P3 (EN 143) respirator cartridges