I5907

Pyrophosphatase, Inorganic from Escherichia coli

recombinant, expressed in E. coli, lyophilized powder, ≥90%, ≥800 units/mg protein

• 동의어(들): Inorganic Pyrophosphatase, Pyrophosphate phosphohydrolase
• CAS Number: 9024-82-2
• EC 번호: 3.6.1.1 (BRENDA, IUBMB)
• EC Number: 232-784-7
• MDL number: MFCD00131379
• UNSPSC 코드: 12352204
• NACRES: NA.32

속성

• 재조합: expressed in E. coli
• Quality Level: 200
• 분석: ≥90%
• 양식: lyophilized powder
• 특이 활성도: ≥800 units/mg protein
• 분자량: hexamer subunit mol wt 20 kDa
• 저장 온도: −20°C

설명

일반 설명

Pyrophosphatase from E coli (E-PPase) has a broader pH optimum and contains four divalent cations per subunit. It comprises 175 amino acids with additional aspartate residue in the active site cavity. Structurally E-PPase is a homohexamer with six identical 20 kDa subunits. Magnesium is a cofactor for E-PPase.

애플리케이션

Inorganic pyrophosphatase (PPase) is a ubiquitous enzyme catalyzing the reaction PP_i + H₂O → 2P_i.
It plays an important role in protein, RNA, and DNA synthesis.

Pyrophosphatase, Inorganic from Escherichia coli has been used as a component of transcription buffer.

Pyrophosphatase, inorganic from Escherichia coli has been used in assay for conjugation of ubiquitin and ubiquitin-like proteins. It has been used for one-pot three-enzyme system for synthesis of Lewis x and sialyl Lewis x antigens.

생화학적/생리학적 작용

Pyrophosphatase from E coli (E-PPase) is an essential enzyme in yeast and bacteria The active site residues are crucial for binding to magnesium.

기타 정보

A homohexameric protein containing 175 amino acid residues per subunit, its activity is Mg²⁺ dependent. It is a relatively thermostable protein.

단위 정의

One unit will release 1.0 μmole of inorganic orthophosphate per minute at pH 9 at 25 °C.

물리적 형태

Lyophilized powder in Tris-buffered salts containing protease inhibitors

안전성 정보

• 픽토그램: GHS07
• 신호어: Warning
• 유해 및 위험 성명서: H315,H319,H335
• 예방조치 성명서: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)