추천 제품
생물학적 소스
mouse
Quality Level
결합
unconjugated
항체 형태
ascites fluid
항체 생산 유형
primary antibodies
클론
SY5, monoclonal
포함
15 mM sodium azide
종 반응성
canine, gorilla, owl monkey, pig, human
기술
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunohistochemistry (frozen sections): suitable
immunoprecipitation (IP): suitable
indirect ELISA: suitable
indirect immunofluorescence: 1:100 using formalin-fixed, paraffin-embedded human skin sections
western blot: suitable
동형
IgG1
UniProt 수납 번호
배송 상태
dry ice
저장 온도
−20°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... IVL(3713)
일반 설명
Involucrin is a soluble precursor protein that regulates terminal differentiation in keratinocytes. This protein is initially present in the cytosol component of keratinocytes and later associates with membrane proteins. Involucrin is expressed in squamous cell cancer and its expression is down-regulated by HPV 16 oncoproteins . Monoclonal Anti-Involucrin is specific for involucrin in primate and non-primate mammals. In cultured human keratinocytes, the antibody stains a 120 kDa band by immunoblot under denaturing-reducing conditions. However, in other cells the antibody binds to involucrin protein in varied sizes: 170 kDa in human breast adenocarcinoma cell line MCF-7, a doublet of approx. 115 and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet at approx 105 kDa in pig. The antibody does not bind to mouse epidermis; hence the antibody can be used to study human xenografts in nude mice. The antibody stains the upper spinous and granular layers in human skin and the cytoplasm of suprabasal terminally differentiated keratinocytes in stratified colonies .
특이성
The antibody detects involucrin in a range of primate and nonprimate mammals in a variety of techniques. In immunoblotting of cultured human keratinocytes, the antibody stains a 120 kDa band under denaturing and reducing conditions. However, in other systems, the antibody stains the involucrin protein in a variety of sizes: 170 kDa in human breast adenocarcinoma cell line MCF-7, a doublet of approximately 115 and 150 kDa in gorilla and owl monkey, 66 kDa in dog, and a doublet of ~105 kDa in pig. The antibody does not react with mouse epidermis, permitting use in studies of human xenografts in nude mice. The antibody stains the upper spinous and granular layers in human skin and the cytoplasm of suprabasal terminally differentiated keratinocytes in stratified colonies.
면역원
human involucrin.
애플리케이션
Monoclonal Anti-Involucrin antibody is suitable for use in immunofluorescence at 1:100 dilutions using keratinocytes or for indirect immunofluorescence using methanol fixed mouse embryonic fibroblasts-keratinocytes co-cultures . The antibody may also be used for western blot (1:1000) and immunohistochemical analysis . Additionally, the product can be useful for indirect ELISA, immunoblotting, immunoprecipitation and immunocytochemistry.
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
10 - Combustible liquids
WGK
nwg
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
Joan Manils et al.
Oncotarget, 6(26), 22375-22396 (2015-06-20)
TREX2 is a 3'-DNA exonuclease specifically expressed in keratinocytes. Here, we investigated the relevance and mechanisms of TREX2 in ultraviolet (UV)-induced skin carcinogenesis. TREX2 expression was up-regulated by chronic UV exposure whereas it was de-regulated or lost in human squamous
Romain Jugé et al.
Scientific reports, 6, 35065-35065 (2016-10-13)
UV irradiation is a major environmental factor causing skin dryness, aging and cancer. UVB in particular triggers cumulative DNA damage, oxidative stress and mitochondrial dysfunction. The objective of our study was to provide both qualitative and quantitative analysis of how
Z Suo et al.
Histopathology, 23(1), 45-54 (1993-07-01)
The expression of cytokeratins (CK) 1, 4, 5/6, 8, 13, 18, 19 and 20 and involucrin in 42 cases of squamous cell carcinomas from various locations was examined. The tumours expressed CK5/6 in 55%, CK8 in 76%, CK13 in 43%
Rui Jorge Nobre et al.
Journal of virology, 83(7), 2907-2916 (2009-01-21)
The genome organization of the novel human papillomavirus type 108 (HPV108), isolated from a low-grade cervical lesion, deviates from those of other HPVs in lacking an E6 gene. The three related HPV types HPV103, HPV108, and HPV101 were isolated from
Jun Dai et al.
PloS one, 8(7), e70392-e70392 (2013-08-08)
RORα is a retinoid-related orphan nuclear receptor that regulates inflammation, lipid metabolism, and cellular differentiation of several non-epithelial tissues. In spite of its high expression in skin epithelium, its functions in this tissue remain unclear. Using gain- and loss-of-function approaches
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