L2005

Lactoperoxidase from bovine milk

lyophilized powder (essentially salt-free), ≥200 units/mg protein

• 동의어(들): Donor:hydrogen peroxide oxidoreductase, Peroxidase
• CAS Number: 9003-99-0
• EC 번호: 1.11.1.7 (BRENDA, IUBMB)
• EC Number: 232-668-6
• MDL number: MFCD00131484
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bovine milk
• Quality Level: 200
• 양식: lyophilized powder (essentially salt-free)
• 특이 활성도: ≥200 units/mg protein
• 흡수비: A_{412/280 nm} 0.7-0.9
• UniProt 수납 번호: P80025
• 저장 온도: −20°C
• SMILES string: [O+H2]O[O-]
• InChI: 1S/H2O3/c1-3-2/h1-2H
• InChI key: JSPLKZUTYZBBKA-UHFFFAOYSA-N
• 유전자 정보: cow ... LPO(280844)

설명

일반 설명

Lactoperoxidase is a major enzyme present in bovine milk and belongs to the peroxidase family. It is present in both plants and animals. The enzyme structure comprises a single polypeptide chain made up of 612 amino acid residues.

애플리케이션

Lactoperoxidase from bovine milk has been used as a standard for milk lactoperoxidase to study the role of H₂O₂ in the inhibition of Staphylococcus aureus growth by Lactococcus garvieae in the presence of lactoperoxidase in raw milk. It has also been used in lactoperoxidase-mediated iodination to prepare radiolabeled peptides.

생화학적/생리학적 작용

Lactoperoxidase catalyzes the oxidation of iodide to iodine by hydrogen peroxide. This activity provides a gentle, specific alternative to chloramine T for the radioiodination of proteins and DNA.

Lactoperoxidase contributes to the antimicrobial system of milk by inactivating a wide range of micro-organisms. This lactoperoxidase-mediated antimicrobial system is also identified in human secretions such as tear-fluid, saliva, and milk. Lactoperoxidase catalyzes the oxidation of molecules by releasing H2O2. The product exhibits antimicrobial activity.

단위 정의

One unit will oxidize 1.0 μmole of 2,2′-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) at pH 5.5 at 25 °C.

분석 메모

Protein determined by Lowry method.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)