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Merck

L2880

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O55:B5

purified by phenol extraction

동의어(들):

LPS

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About This Item

CAS Number:
EC Number:
MDL number:
UNSPSC 코드:
12352201
NACRES:
NA.25
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생물학적 소스

Escherichia coli (O55:B5)

Quality Level

양식

lyophilized powder

정제법

phenol extraction

기술

cell based assay: suitable

불순물

<3% Protein (Lowry)

색상

off-white to tan

solubility

water: soluble

응용 분야

cell analysis
clinical research
life science and biopharma

배송 상태

ambient

저장 온도

2-8°C

일반 설명

Lipopolysaccharides (LPS) are glycolipids, a combination of carbohydrate units linked with single or multiple fatty acids that are found in the cell wall of Gram-negative bacteria. The LPS is an essential component of the outer membrane and the LPS structure is composed of lipid A, a glucosamine-based phospholipid, a short-core oligosaccharide, and an O-antigen, a distal polysaccharide.

애플리케이션

Lipopolysaccharide from Escherichia coli O55:B5 has been used:
  • to stimulate hepatocytes and non-parenchymal cells
  • to stimulate the spleen and study the expression of the Malitaf gene in the fish blunt snout bream
  • as part of the concanavalin A (ConA) and D-galactosamine/lipopolysaccharide (D-GalN/LPS) model of fulminant hepatitis to study the effect of the natural compound hesperetin
  • to check the cell viability of human chorionic villi-derived mesenchymal stem cells (hCMSCs) by methyl thiazolyl tetrazolium (MTT) assay
  • to induce oligodendrocyte toxicity to evaluate the neuroprotective effects of few compounds
  • to induce sarcopenia in rats to evaluate the antioxidant effects of curcumin-loaded hydrophobic surface-modified hydroxyapatite
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

생화학적/생리학적 작용

Lipopolysaccharides (LPS) sets up a protective permeability barrier that blocks the entry of antibiotics and bile salts, which make Gram-negative bacteria innately resistant. LPS is involved in bacterial pathogenicity as the host immune system primarily encounters the LPS component of the bacteria. LPS is an endotoxin and a highly immunogenic antigen that can enhance the immune system. The lipid A of LPS stimulates host cells via toll-like receptors 4 (TLR4).

기타 정보

For additional information on our range of Biochemicals, please complete this form.
For optimal reconstitution of LPS products, an intensive vortex is required before use, regardless of the container type. Ensure that the storage solution concentration is at least 1 mg/ml. While borosilicate (silanized) containers are recommended, polypropylene (PP) plastic containers may also be used.
To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.


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픽토그램

Skull and crossbones

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 2 Oral

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 3

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable

개인 보호 장비

Eyeshields, Gloves, type N95 (US)



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