M3769

Medium 199

Modified, with Earle′s salts, without L-glutamine, sodium bicarbonate, and phenol red, powder, suitable for cell culture

• 동의어(들): M199 Medium, TCM 199
• UNSPSC 코드: 12352207
• NACRES: NA.71

속성

• Quality Level: 500
• 양식: powder
• 기술: cell culture | mammalian: suitable
• 성분: L-glutamine: no; phenol red: no; HEPES: no; sodium pyruvate: no; Earle’s salts (5% CO₂): yes; NaHCO₃: no
• 배송 상태: ambient
• 저장 온도: 2-8°C

설명

일반 설명

Medium 199 is a combination of vitamins, amino acids, and other factors that were originally developed as a completely defined media formulation for the culture of primary explants. This medium, when properly supplemented has broad applicability, particularly for non-transformed cells. Medium 199 is widely used for vaccine production, in vitro cultivation of primary pancreatic explants, and lens tissues.

애플리케이션

Medium 199 has been used:

• to culture the primary human umbilical vein endothelial cells (HUVECs) for use in cell viability assay
• to flush the uterine horn of gilts to recover day 12 conceptuses from uteri
• to culture the peritoneal cells and immortalized human mesothelial cells (MeT-5A and ATCC-CRL-9444)

수량

Formulated to contain 9.4 grams of powder per liter of medium.

재구성

Supplement with 0.1 g/L L-glutamine, 2.2 g/L sodium bicarbonate.

안전성 정보

• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable