M4439

Anti-c-Myc antibody, Mouse monoclonal

clone 9E10, purified from hybridoma cell culture

• 동의어(들): Anti-c-Myc
• UNSPSC 코드: 12352203
• NACRES: NA.56

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified from hybridoma cell culture
• 항체 생산 유형: primary antibodies
• 클론: 9E10, monoclonal
• 양식: buffered aqueous solution
• 분자량: 62 kDa
• 종 반응성: human
• 농도: ~2 mg/mL
• 기술: immunocytochemistry: suitable; immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable; immunoprecipitation (IP): suitable; microarray: suitable; western blot: 1:5000 using c-myc tagged fusion protein
• UniProt 수납 번호: P01106
• 배송 상태: dry ice
• 저장 온도: −20°C
• 유전자 정보: human ... MYC(4609)

설명

일반 설명

The mouse Anti-c-MYC monoclonal antibody recognizes an epitope located within a sequence (EQKLISEEDL) of the product of the human oncogene c-myc.

c-Myc (v-myc avian myelocytomatosis viral oncogene homolog) is a transcription factor, which is coded by MYC gene. It was first described as a homolog of an avian retroviral oncogene. It belongs to the family of transcription factors which are characterized by basic region helix-loop-helix/leucine zipper. Along with N-Myc and L-Myc, it forms the MYC proto-oncogene family. It forms a heterodimer with MAX, and this heterodimer binds to the E-boxes/CACGTG sequence motif on DNA. This gene is localized to human chromosome 8q24.

면역원

synthetic peptide of the human p62^c-Myc protein.

애플리케이션

Monoclonal Anti-c-Myc antibody produced in mouse is suitable for immunoprecipitation, immunoblotting, ELISA, immunofluorescence, microarray and electron microscopy. It has also been used in ChIP (Chromatin Immunoprecipitation).

생화학적/생리학적 작용

v-myc avian myelocytomatosis viral oncogene homolog belongs to MYC family. When overexpressed these proteins can cause DNA double-stand breaks (DSBs) and genome instability. This transcription factor MYC helps in regulating long noncoding RNAs (lncRNAs) which has been implicated in cancer cell proliferation and tumorigenesis. Its overexpression is also associated with various cancers, and in particular B cell lymphomas. Increased expression of c-MYC has a negative effect on the ability of B cell lymphomas to functionally present Ags/peptides to CD4⁺ T cells. This protein exerts suppressive effects at several critical checkpoints in Ag presentation. It is associated with Burkitt lymphoma and a group of diffuse large B-cell lymphoma (DLBCL).

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

제조 메모

Working dilution: 1:5000

기타 정보

2024 CiteAb Award Winner for Supplier Succeeding in Parkinson′s Research

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable