M5650
Minimum Essential Medium Eagle
With non-essential amino acids, Earle′s salts and sodium bicarbonate, without ʟ-glutamine, liquid, sterile-filtered, suitable for cell culture
동의어(들):
EMEM, MEM
Product Name
Minimum Essential Medium Eagle, With Earle′s salts, non-essential amino acids and sodium bicarbonate, without L-glutamine, liquid, sterile-filtered, suitable for cell culture
Quality Level
무균
sterile-filtered
양식
liquid
기술
cell culture | mammalian: suitable
불순물
endotoxin, tested
성분
phenol red: yes
NaHCO3: yes
L-glutamine: no
sodium pyruvate: no
HEPES: no
Earle’s salts (5% CO2): yes
배송 상태
ambient
저장 온도
2-8°C
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일반 설명
Minimum Essential Medium Eagle (MEM) is a synthetic cell culture media, developed by Harry Eagle. It has higher concentrations of amino acids so the medium more closely approximates the protein composition of cultured mammalian cells. Optional supplementation of non-essential amino acids to the formulations that incorporate either Hanks′ or Earle′s salts has enhanced the uses of this medium. This formulation has been further modified by the optional elimination of calcium to permit the growth of cells in suspension culture. MEM can be used for the cultivation of a wide variety of cells grown in monolayers.
애플리케이션
Minimum Essential Medium Eagle has been used to culture:
This medium may be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.
- HeLa cells
- Madin-Darby canine kidney (MDCK) cells
- chondrosarcoma cell line
This medium may be preferred for the growth of adherent cells in the presence of reduced serum or as a base for the development of a serum-free medium.
재구성
Supplement with 0.292 g/L L-glutamine.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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