M8195

Monoclonal Anti-phospho-MDMX (pTyr⁹⁹) antibody produced in mouse

~2 mg/mL, clone PH-MDMX-169, purified immunoglobulin, buffered aqueous solution

• 동의어(들): Anti-HDM4, Anti-HDMX, Anti-MDM4, Anti-MGC132766, Anti-MRP1
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: PH-MDMX-169, monoclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~80 kDa
• 종 반응성: human
• 포장: antibody small pack of 25 μL
• 농도: ~2 mg/mL
• 기술: indirect ELISA: suitable; indirect immunofluorescence: suitable; western blot: 1-2 μg/mL using total cell extract of HEK-293T cells co-transfected with human MDMX and a specific kinase
• 동형: IgG2b
• UniProt 수납 번호: O15151
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: phosphorylation (pTyr99)
• 유전자 정보: human ... MDM4(4194)

설명

일반 설명

MDMX is a nuclear protein and the gene encoding it is localized on human chromosome 1q32.1.

Monoclonal Anti-phospho-MDMX (pTyr99) (mouse IgG2b isotype) is derived from the hybridoma PH-MDMX-169 produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice. Mouse double minute 4 (MDM4) or MDMX comprises p53 binding domain, central acidic region, zinc and interesting new gene (RING) finger domain and is a phosphorylated protein. MDM4 gene is mapped to human chromosome 1q32.1.

특이성

Monoclonal Anti-phospho-MDMX (pTyr99) recognizes human phosphorylated MDMX (pTyr99) (~ 80 kDa). The antibody does not recognize the non-phosphorylated MDMX, which is produced when MDMX is expressed with a kinase mutant.

면역원

synthetic phosphopeptide corresponding to amino acids 93-106 (pTyr⁹⁹) of human MDMX.

애플리케이션

Monoclonal Anti-phospho-MDMX (pTyr99) antibody produced in mouse may be used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunofluorescence
• immunoblotting

생화학적/생리학적 작용

MDMX associates with tumor suppressor p53 and inhibits its activity. It is upregulated in many tumors.

Studies began to unravel the regulation of murine double minute X (MDMX) in response to stress conditions, involving post-translational modifications and protein-protein interactions.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

저장 및 안정성

For extended storage, freeze at -20 °C in working aliquots. Repeated freezing and thawing, or storage in “frost-free” freezers, is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discard if not used within 12 hours.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)