MAK137

Phospholipase D Activity Assay Kit

sufficient for 100 colorimetric or fluorometric tests

• 동의어(들): PLD Activity Assay Kit
• UNSPSC 코드: 12161503
• NACRES: NA.84

속성

• 사용: sufficient for 100 colorimetric or fluorometric tests
• 검출 방법: colorimetric; fluorometric
• 관련 질환(들): cancer
• 저장 온도: −20°C
• 유전자 정보: human ... PLD1(5337), PLD2(5338), PLD3(23646), PLD4(122618), PLD5(200150), PLD6(201164) ; mouse ... PLD1(18805), PLD2(18806), PLD3(18807), PLD4(104759), PLD5(319455), PLD6(194908) ; rat ... PLD1(25096), PLD2(25097), PLD3(361527), PLD4(362792), PLD5(289270), PLD6(287366)

설명

일반 설명

Phospholipase D (PLD) catalyzes the hydrolysis of the phosphodiester bond of glycerophospholipids to generate phosphatidic acid and choline. It is ubiquitously expressed and is implicated in immune function and cancer inhibition. Abnormalities in PLD expression have been associated with human cancers such as breast, renal, gastric and colorectal cancer.

애플리케이션

Phospholipase D (PLD) Activity Assay Kit has been used to measure PLD activity in the liver and lysosomal fractions of the mice liver.

Suitable for the detection of phospholipase D activity in biological samples. This kit can be used to study the effects of various compounds on phospholipase D activity.

특징 및 장점

Compatible with high-throughput handling systems.

적합성

Suitable for the quantitative determination of phospholipase D activity and evaluation of drug effects on phospholipase D metabolism.

원리

In this assay, PLD hydrolyzes phosphatidylcholine to choline, which is determined using choline oxidase resulting in a colorimetric (570 nm)/fluorometric (λex = 530/λem = 585 nm) product, proportional to the PLD activity in the sample. Unit definition: one unit of PLD catalyzes the formation of 1 mmole choline per minute under the assay conditions (pH 7.4).

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334,H412
• 예방조치 성명서: P261 - P273 - P284 - P501
• Hazard Classifications: Aquatic Chronic 3 - Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): 188.6 °F - closed cup
• Flash Point (°C): 87 °C - closed cup