MAK193

Citrate Synthase Activity Assay Kit

Sufficient for 100 Colorimetric tests

• 동의어(들): Citrate Synthase Test Kit
• EC Number: 232-821-7
• UNSPSC 코드: 12161503
• NACRES: NA.84

속성

• 검출 방법: colorimetric
• 관련 질환(들): endocrinological disorders, diabetes; cancer
• 저장 온도: −20°C
• 유전자 정보: human ... CS(1431) ; mouse ... CS(12974) ; rat ... CS(170587)

설명

일반 설명

Citrate Synthase (CS; EC 2.3.3.1) is a mitochondrial enzyme found in all cells capable of oxidative metabolism. It catalyzes the synthesis of citrate from oxaloacetate in the Krebs tricarboxylic acid cycle.1,2 CS is involved in energy production, lipogenesis, and cholesterologenesis, and its activity follows a circadian pattern.

애플리케이션

Citrate Synthase Activity Assay Kit has been used to measure the activity of the enzyme citrate synthase in samples.

적합성

Suitable for the detection of citrate synthase activity in a variety of biological samples such as cell and tissue culture supernatants and purified mitochondria.

원리

The Citrate Synthase Activity Assay kit provides a simple and direct procedure for measuring the activity of CS in a variety of tissues, cell cultures (adherent or suspension), and purified mitochondria. CS activity is determined using a coupled enzyme reaction, which results in a colorimetric (412 nm) product proportional to the enzymatic activity present. One unit of CS is the amount of enzyme that generates 1.0 mmole CoA per minute at 25 °C and pH 7.2.

키트 구성품 전용

• CS Assay Buffer
• CS Substrate Mix
• CS Developer
• GSH Standard, Reduced
• CS Positive Control

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H319,H334
• 예방조치 성명서: P261 - P264 - P280 - P284 - P305 + P351 + P338 - P337 + P313
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3