MWGF1000

Gel Filtration Markers Kit for Protein Molecular Weights 29,000-700,000 Da

• 동의어(들): Gel Filtration Markers Kit, Gel Filtration Protein Ladder, Protein Molecular Weight Marker Kit
• UNSPSC 코드: 12352200
• NACRES: NA.25

속성

• 양식: liquid
• Quality Level: 200
• 기술: liquid chromatography (LC): suitable
• 저장 온도: −20°C

설명

일반 설명

Gel filtration chromatography is a well-known technique for measuring the size and molecular weight of proteins. It separates proteins based on their ability to diffuse into the pores of the resin. Larger proteins have more difficulty entering the resin pores, so they flow through the column more quickly than smaller proteins. As a result, the proteins are eluted from the column in order of decreasing molecular weight.

애플리케이션

Gel Filtration Markers Kit for Protein Molecular Weights 29,000-700,000 Da has been used:

• for protein purification and analyses (gel-filtration experiments)
• for size-exclusion chromatography/gel filtration chromatography of synaptoneurosome extract
• for glycerol gradient centrifugation
• to study glucocorticoid-induced lymphocytolysis as a model system for apoptosis within the immune system
• to isolate Polygonatum odoratum lectin, which showed remarkable anti-HSV-II activity towards Vero cells, cytotoxicity towards human melanoma A375 cells and induced apoptosis in a caspase-dependent manner

생화학적/생리학적 작용

The method described in the bulletin for determining molecular masses using gel filtration chromatography is a modified version of existing published techniques. The protein standards included in this kit may be compatible with other chromatographic systems like high-performance liquid chromatography (HPLC). However, certain buffer systems may affect the elution volumes of albumin and carbonic anhydrase. The proteins in this kit have a molecular mass range spanning from 29 kDa to 699 kDa.

기타 정보

• For research use only. Not for drug, household, or other uses.
• Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.
• Please refer to the product information sheet to learn more about reagents, storage and stability, and procedure.

키트 구성품 역시 별도로 이용 가능함

• A8531 Albumin, bovine serum, 66,000 MW 1 mL/vial
• A8656 Alcohol Dehydrogenase, yeast, 150,000 MW 1 mL/vial
• A8781 β-Amylase, sweet potato, 200,000 MW 1 mL/vial
• A3660 Apoferritin, horse spleen, 443,000 MW 1 mL/vial
• D4772 Blue dextran, 2,000,000 MW 1 mL/vial
• C7025 Carbonic Anhydrase, bovine erythrocytes, 29,000 MW 1 mL/vial
• T9145 Thyroglobulin, bovine, 669,000 MW 1 mL/vial

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 10 - Combustible liquids
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable