O6014

Anti-O-GlcNAc Transferase (TI-14) antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

• 동의어(들): Anti-O-linked N-Acetylglucosamine Transferase, Anti-OGT
• MDL number: MFCD07370422
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: IgG fraction of antiserum
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 분자량: antigen 110 kDa
• 종 반응성: mouse, human, rat
• 기술: indirect immunofluorescence: 1:50-1:100 using A549 human lung carcinoma cells fixed with paraformaldehyde/triton; western blot: 1:1,000-1:2,000 using HeLa cell nuclear extracts
• UniProt 수납 번호: O15294
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... OGT(8473) ; mouse ... Ogt(108155) ; rat ... Ogt(26295)

설명

일반 설명

O-GlcNAc transferase (OGT) catalyzes the addition of an N-acetylglucosamine residue to the amino acids serine or threonine. The enzyme is an homotrimer consisting of three subunits of 110 kDa each, with multiple tetratricopeptide (TPR) repeats.

면역원

synthetic peptide corresponding to amino acids 1024-1037 of human O-GlcNAc transferase, conjugated to KLH via an N-terminal added cysteine residue.

애플리케이션

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

생화학적/생리학적 작용

O-linked β-N-acetyl glycosamine (O-GlcNAc) is involved in repressing transcription. O-GlcNAc transferase (OGT) interacts with a histone deacetylase complex by binding to the co repressor Sin3A. This interaction leads to the repression of transcription, after transcription factors and RNA polymerase II are modified by addition of O-GlcNAc. O-GlcNAc modification reversibly inhibits proteosomal function in an ubiquitin-independent fashion.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, and 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)