OGS10
PSF-CMV-KAN - KANAMYCIN RESISTANCE PLASMID
plasmid vector for molecular cloning
동의어(들):
cloning vector, expression vector, molecular cloning vector, plasmid, plasmid vector, snapfast vector, vector
About This Item
추천 제품
양식
buffered aqueous solution
분자량
size 4245 bp
박테리아 선정
kanamycin
복제개시점
pUC (500 copies)
펩타이드 절단
no cleavage
촉진제
Promoter name: CMV
Promoter activity: constitutive
Promoter type: mammalian
리포터 유전자
none
배송 상태
ambient
저장 온도
−20°C
1 of 4
이 품목 | WHA7402009 | WHA7404001 | WHA7404002 |
---|---|---|---|
pore size 0.45 μm | pore size 0.2 μm | pore size 0.45 μm | pore size 0.45 μm |
manufacturer/tradename Whatman 7404-009, Whatman Article No. 28420772 (US reference) | manufacturer/tradename Whatman 7402-009, Whatman Article No. 28420768 (US reference) | manufacturer/tradename Whatman 7404-001, Whatman Article No. 28420769 (US reference) | manufacturer/tradename Whatman 7404-002, Whatman Article No. 28420770 (US reference) |
material Nylon membrane | material Nylon membrane | material Nylon membrane | material Nylon membrane |
sterility non-sterile | sterility non-sterile | sterility non-sterile | sterility non-sterile |
packaging pkg of 50 ea | packaging pkg of 50 ea | packaging pkg of 100 ea | packaging pkg of 100 ea |
diam. 90 mm | diam. 90 mm | diam. 13 mm | diam. 25 mm |
일반 설명
Promoter Expression Level: This plasmid contains the mammalian CMV promoter to drive gene expression. We have tested all of our mammalian promoters in a range of cell types and CMV is consistently the strongest in those we have studied. However there are many reports of the CMV promoter demonstrating silencing by methylation in long-term culture. For this reason we stock a range of other promoters that are compatible with this plasmid and are available on request.
애플리케이션
Multiple cloning site notes: There are a few important sites within the MCS. These include the NcoI site the XbaI site and the BsgI and BseRI sites. The NcoI site contains a start codon that is immediately downstream of both a Kozak and Shine-Dalgarno ribosomal binding site. These allow for optimal positioning of genes when the start codon is placed in this location. If this is not required and you wish to use a downstream site for gene cloning you can remove the NcoI site by cleaving the plasmid with KpnI.
The XbaI site contains a stop codon. This stop codon is positioned in a specific position in relation to the BsgI and BseRI sites that are immediately downstream. When either BseRI or BsgI cleave the plasmid they produce a TA overhang from the stop codon in the XbaI site that is compatible with all of our peptide tag plasmids cut with the same sites. BseRI and BsgI sites are non-palindromic and cleave a defined number of bases away from their binding site.
Whenever we clone a gene into our multiple cloning site we always position the start and stop codon in the same positions in the MCS. If the start and ends of the genes are not compatible with NcoI and XbaI we extend the sequence to the nearest external sites but keep the start and stop codons locations consistent.
서열
분석 메모
관련 제품
Storage Class Code
12 - Non Combustible Liquids
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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