P0194

Protein Kinase Cζ isozyme human

≥75% (SDS-PAGE), recombinant, expressed in baculovirus infected insect cells, buffered aqueous solution

• 동의어(들): Ca²⁺-activated phospholipid-dependent serine-threonine kinase ζ isozyme human, PKCζ human
• EC 번호: 2.7.1.37 (BRENDA, IUBMB)
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 재조합: expressed in baculovirus infected insect cells
• Quality Level: 200
• 분석: ≥75% (SDS-PAGE)
• 양식: buffered aqueous solution
• enzyme activity: >800 units/mg protein
• 분자량: 76-80 kDa by SDS-PAGE
• UniProt 수납 번호: Q05513
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... PRKCZ(5590)

설명

생화학적/생리학적 작용

PKCζ is a downstream enzyme in insulin signaling pathways.

Protein Kinase C (PKC) is a serine/threonine kinase that is activated intracellularly by signal transduction pathways that produce DAG from phosphatidylinositol diphosphate (PIP₂) and phosphatidylcholine (PC) through the action of various activated phospholipases. Phorbol esters also stimulate PKC. At least 11 PKC isozymes have been identified that differ in primary structure, tissue distribution, subcellular localization, response to extracellular signals, and substrate specificity. The isozymes can be grouped into three subfamilies. Members of the first family require Ca²⁺ and phospholipid and include PKCα, βI, βII, and γ. Members of the second family are phospholipid-dependent but Ca²⁺-independent, and include PKCδ, ε, η, and θ. Members of the third family are not activated by either DAG or phorbol esters and include PKCξ, μ, and ι.
Phosphorylation appears to be an important mechanism of regulation of all PKCs. PKC plays a role in the regulation of cell transformation, growth, differentiation, ruffling, vesicle trafficking, apoptosis and gene expression.

적합성

PKC ε can transfer 1100 nmole of phosphate to PKC ε substrate peptide per minute per mg of total protein at 30 °C.

단위 정의

One unit will transfer 1 nanomole of phosphate to PKC epsilon substrate peptide per minute at pH 7.5 at 30 deg C.

물리적 형태

Solution in 20 mM HEPES, pH 7.5; 2 mM EDTA, 2 mM EGTA, 5 mM DTT, 250 mM NaCl, 0.05% Triton X-100, and 50% glycerol.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)