P1944

Phenol:Chloroform:Iso-amyl alcohol (125:24:1)

• 동의어(들): phenol chloroform RNA extraction
• UNSPSC 코드: 12190000
• NACRES: NA.52

속성

• Grade: Molecular Biology
• Quality Level: 200
• 분석: ≥99.0%
• 양식: liquid
• pH: 4.3-4.7
• 응용 분야: life science and biopharma
• 저장 온도: 2-8°C

설명

일반 설명

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) is used for the purification of total RNA from mixtures of crude cell extracts and can eliminate the need for a time consuming ultracentrifugation step. The addition of phenol, chloroform, and isoamyl can be employed to aid in the separation of lipids and cellular debris, leading to the transfer of these components to the organic phase. Meanwhile, the genomic DNA (gDNA) remains in the aqueous phase, effectively isolating it from the other substances.

애플리케이션

Phenol:Chloroform:Iso-amyl alcohol (125:24:1) has been used:

• for the extraction and purification of RNA.
• to isolate RNA from Arabidopsis siliques.
• to extract proteins and DNA from polysomal and non-polysomal fractions.
• in TRIzol methods to prepare and extract miRNAs from cells, tissue, and whole flies.

안전성 정보

• 픽토그램: GHS06,GHS08,GHS05,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H301 + H311 + H331,H314,H341,H351,H361d,H372,H373,H411
• 예방조치 성명서: P202 - P273 - P280 - P303 + P361 + P353 - P304 + P340 + P310 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2
• 표적 기관: Liver,Kidney, Nervous system,Kidney,Liver,Skin
• Storage Class Code: 6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials
• WGK: WGK 3
• Flash Point (°F): 175.0 °F - closed cup
• Flash Point (°C): 79.44 °C - closed cup
• 개인 보호 장비: Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter