추천 제품
재조합
expressed in E. coli
Quality Level
양식
aqueous ethanol suspension
분석물 화학적 분류
proteins (Immunoglobulins of various mammalian species)
라벨링 범위
~2 mg per mL
기술
affinity chromatography: suitable
Matrix
Sepharose 4B Fast Flow
기질 활성
cyanogen bromide
기질 부착
amino
기질 스페이서
1 atom
저장 온도
2-8°C
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일반 설명
Protein G is an immunoglobulin (IgG)-binding bacterial cell wall protein isolated from group G streptococcal strain, G-148. This protein can be extracted from the cells by papain digestion and purified by the sequential use of ion-exchange chromatography on DEAE-Sephadex, affinity chromatography on Sepharose-coupled human IgG, and gel chromatography on Sephadex G-200. The binding between protein G and various polyclonal and monoclonal IgG is basically pH dependent between 2.8 and 10, with the strongest binding at pH 4 and 5, and weakest at pH 10. It acts as a powerful reagent for the detection of IgG.
P3296-5Ml′s updated product number is GE17-0618-01
P3296-5Ml′s updated product number is GE17-0618-01
애플리케이션
Protein G-Sepharose™ is used in affinity chromatography, protein chromatography, antibody purification and characterization, immunoaffinity matrices, protein A, G and L resins, protein interaction, and purification and detection. Protein G-Sepharose™ has been used to develop a strategy to confirm the presence of anti-erythropoietin neutralizing antibodies in human serum as well as to compare methods for depletion of albumin and IgG from equine serum.
물리적 형태
Suspension in 20% ethanol
제조 메모
Prepared with recombinant streptococcal Protein G from which the albumin-binding region has been genetically deleted
법적 정보
Sepharose is a trademark of Cytiva
신호어
Warning
유해 및 위험 성명서
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 3
Flash Point (°F)
115.0 °F - closed cup
Flash Point (°C)
46.1 °C - closed cup
이미 열람한 고객
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프로토콜
To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.
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