P3738

Protein Kinase G Iβ human

≥95% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf9 cells, buffered aqueous glycerol solution

• 동의어(들): cyclic-Guanosine Monophosphate-dependent Protein Kinase 1β human
• EC 번호: 2.7.11.12 (BRENDA, IUBMB)
• MDL number: MFCD03458230
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in baculovirus infected Sf9 cells
• Quality Level: 200
• 분석: ≥95% (SDS-PAGE)
• 양식: buffered aqueous glycerol solution
• 특이 활성도: ≥1.5 units/mg protein (20-fold stimulation by cGMP (5 μM))
• 분자량: 76 kDa (monomer)
• UniProt 수납 번호: Q13976
• 관련 질환(들): cancer
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: human ... PRKG1(5592)

설명

애플리케이션

Protein Kinase G is a serine/threonine-specific protein kinase that is activated by cGMP. Protein Kinase G Iβ is used to induce apoptosis and inhibit cell proliferation.

생화학적/생리학적 작용

Protein Kinase G Iβ induces apoptosis in certain cell lines such as human breast cancer cell lines MCF-7 and MDA-MB-468. It inhibits cell proliferation and induces apoptosis in colon cancer cell lines.

단위 정의

One unit will phosphorylate 1 micromole of VASPtide(RRKVSKQE) substrate per minute in 10 mM HEPES, pH 7.4, 5 mM MgCl2, 1 mM DTE and 0.2 mM EDTA.

물리적 형태

Solution in 20 mM Tris buffer, pH 7.4, 1 mM EDTA, 1 mM β-mercaptoethanol, 100 mM NaCl, 10 U/ml Trasylol, and 50% glycerol.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable