P4056

Platelet-Derived Growth Factor-BB from rat

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture

• 동의어(들): PDGF-BB
• MDL number: MFCD01094933
• UNSPSC 코드: 12352202
• NACRES: NA.77

속성

• Product Name: Platelet-Derived Growth Factor-BB from rat, PDGF-BB, recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
• 재조합: expressed in E. coli
• Quality Level: 100
• 양식: lyophilized powder
• 효능: 1.62 ng/mL EC₅₀
• 품질: endotoxin tested
• 분자량: ~30 kDa
• 포장: pkg of 5x50 μg; pkg of 50 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 기술: cell culture | mammalian: suitable
• 불순물: ≤1 EU/μg
• UniProt 수납 번호: P31240
• 저장 온도: −20°C
• 유전자 정보: rat ... Pdgfb(24628)

설명

일반 설명

Platelet-derived growth factor (PDGF) is a 30 kDa cationic polypeptide and consists of two disulphide linked chains A and B. PDGF is expressed as hetero and homodimers : PDGF-AB FDGF-AA, PDGF-BB, PDGF-CC and PDGF-DD.

애플리케이션

Platelet-Derived Growth Factor-BB from rat has been used as a growth supplement in mouse embryonic fibroblast culture, motoneuron-muscle co-culture and adult spinal cord neurons culture.

생화학적/생리학적 작용

Platelet-derived growth factor (PDGF) elicits multifunctional actions with a variety of cells. It is mitogenic to mesoderm-derived cells, such as dermal and tendon fibroblasts, vascular smooth muscle cells, glial cells, and chondrocytes. PDGF is a potent chemoattractant and activator of neutrophils, monocytes, and fibroblasts. PDGF activates smooth muscle relocalization and multiplication. PDGF mediated increased smooth muscle muscle proliferation might be associated with gene regulation. It increases the synthesis of phospholipids, cholesterol esters, glycogen and prostaglandins, and modulates LDL (low density lipoprotein) receptor binding.

기타 정보

Dimer of the B chain of rat PDGF.

물리적 형태

Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% trifluoroacetic acid containing 2.5 mg bovine serum albumin.

분석 메모

The biological activity is measured by its ability to stimulate ³H-thymidine incorporation in quiescent NR6R-3T3 fibroblasts.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)