P4439

Phosphatase, Alkaline from porcine kidney

lyophilized powder, ≥100 DEA units/mg protein

• 동의어(들): Orthophosphoric-monoester phosphohydrolase (alkaline optimum)
• CAS Number: 9001-78-9
• EC 번호: 3.1.3.1 (BRENDA, IUBMB)
• EC Number: 232-631-4
• MDL number: MFCD00131849
• UNSPSC 코드: 12352204
• eCl@ss: 42010105
• NACRES: NA.54

속성

• 양식: lyophilized powder
• Quality Level: 200
• 특이 활성도: ≥100 DEA units/mg protein
• 구성: Protein, ≥50%
• 저장 온도: −20°C

설명

애플리케이션

Alkaline phosphatase is used for conjugation to antibodies and other proteins for ELISA, Western blotting, and histochemical detection. Alkaline phosphatase is also used to prevent DNA self ligation and for radiolabeling.

생화학적/생리학적 작용

Alkaline phosphatase is a hydrolase enzyme that dephosphorylates molecules such as nucleotides, proteins and alkaloids.

포장

Package size based on DEA units

단위 정의

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate per min at pH 9.8 at 37 °C.

물리적 형태

Lyophilized fromTris-HCl buffer, with magnesium chloride and zinc chloride, pH 8.0

분석 메모

Protein determined by biuret.

안전성 정보

• 픽토그램: GHS08
• 신호어: Danger
• 유해 및 위험 성명서: H334
• 예방조치 성명서: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)