P6248

Anti-Parkin antibody, Mouse monoclonal

clone PRK8, purified from hybridoma cell culture

• 동의어(들): Anti-AR-JP, Anti-LPRS2, Anti-PARK2, Anti-PDJ
• MDL number: MFCD02263896
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: PRK8, monoclonal
• 양식: buffered aqueous solution
• 종 반응성: mouse, rat, hamster, human
• 농도: ~2 mg/mL
• 기술: microarray: suitable; western blot: 0.25-0.5 μg/mL using using rat brain cytosolic S1 extract
• 동형: IgG2b
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... PARK2(5071) ; mouse ... Park2(50873) ; rat ... Park2(56816)

설명

일반 설명

Monoclonal Anti- Parkin (mouse IgG2b isotype) is derived from the hybridoma PRK8 produced by the fusion of mouse myeloma cells (SP2 cells) and splenocytes from BALB/c mice immunized with recombinant human Parkin. Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. The parkin gene encodes a protein of 465 amino acid residues with moderate similarity to ubiquitin at the amino-terminus and a ring in between ring fingers (IBR)-ring-finger motif at the carboxyl-terminus. The gene has 12 exons.

Parkin is an ubiquitin-protein ligase expresses in many tissues such as brain, testis, skeletal muscle and heart. It is a RING-type E3 ubiquitin-protein ligase that facilitates the ubiquitination of misfolded proteins and protects from neurotoxic effect caused by these proteins. Monoclonal anti-parkin antibody can be used in microarray and immunoprecipitation assays. It can also be used in western blotting. Mouse anti-parkin antibody reacts specifically with parkin (50kD approx) of rat, hamster, mouse and human.

면역원

recombinant human Parkin.

애플리케이션

Monoclonal anti-parkin antibody has been used in:

• Enzyme linked immunosorbent assay (ELISA)
• Immunoblotting
• Immunoprecipitation[63}
• Immunocytochemistry
• to label parkin in pull-down assay

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)