P8279

Protocatechuate 3,4-Dioxygenase from Pseudomonas sp.

lyophilized powder, ≥3 units/mg solid

• CAS Number: 9029-47-4
• EC 번호: 1.13.11.3 (BRENDA, IUBMB)
• MDL number: MFCD00132132
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bacterial (Pseudomonas spp.)
• Quality Level: 200
• 양식: lyophilized powder
• 특이 활성도: ≥3 units/mg solid
• 분자량: ~700 kDa
• 배송 상태: dry ice
• 저장 온도: −20°C

설명

일반 설명

Protocatechuate 3,4-Dioxygenase belongs to the non-heme iron family of enzymes. The active site of the enzyme contains Fe³⁺.

애플리케이션

Protocatechuate 3,4-Dioxygenase(PCD), from Pseudomonas sp., is used for the enzymatic determination of choline esterase when coupled with phydroxybenzoate hydroxylase. It is used to improve organic fluorophore-stability in single-molecule experiments and is used to study the metabolism of protocatechuate in Rhizobiaceae.

The enzyme has been used to create an oxygen scavenging system along with protocatechuate (PCA) and Trolox. The enzyme employs a nonheme iron center that catalyzes the conversion of PCA and molecular oxygen into β-carboxy-cis,cis-muconic acid, while the antioxidant Trolox suppresses slow blinking and photobleaching of cyanine dyes. It has been used in the preparation of imaging buffer along with DMB-BSA (dynein motility buffer-BSA), ATP and protocatechuate in single molecule motility assay.

생화학적/생리학적 작용

Protocatechuate 3,4-Dioxygenase catalyzes the degradation of 3,4-dihydroxybenzoate (protocatechuate) into β-carboxy-cis,cis-muconate.

물리적 특성

Structure : Protein with nonheme iron
Inhibitors : Ag⁺, Hg^++, PCMB
Optimum pH : 9.0
Optimum temperature : 60−65°C
pH Stability : pH 7.0−9.0 (25°C, 72hr)
Thermal stability : below 50°C (pH 6.0, 1hr)

단위 정의

One unit will oxidize 1.0 μmole of protocatechuate to 3-carboxy-cis,cis-muconate per min at pH 7.5 at 37 °C.

물리적 형태

Supplied as lyophilized powder.

분석 메모

Protein determined by biuret.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)