P9582

Polyinosinic–polycytidylic acid potassium salt

with buffer salts, TLR ligand tested

• 동의어(들): Poly (I:C), Poly(I) • Poly(C)
• CAS Number: 31852-29-6
• MDL number: MFCD00131983
• UNSPSC 코드: 41106305
• NACRES: NA.51

속성

• Quality Level: 300
• 분석: ≥99% (less than 1% free nucleotides, TLC)
• 양식: lyophilized powder
• 구성: Poly(I) • Poly(C), 10% (balance buffer salts as sodium chloride and sodium phosphate)
• 저장 조건: desiccated
• 저장 온도: −20°C

설명

애플리케이션

TLR3 recognizes double-stranded RNA and is a major effector of the immune response against viral pathogens. Polyinosinic–polycytidylic acid (Poly(I) • Poly(C)) is a double-stranded homopolymer used as a model RNA to study cell signaling at the level of TLR3. Poly(IC) is a TRIF-dependent toll-like receptor-3 (TLR3) ligand.

생화학적/생리학적 작용

Transfection of Poly (I:C) into NIT-1 cells has been used as a model of intracellular dsRNA-induced β cell apoptosis. Eighteen hours post transfection, 45% of the cells were apoptotic with an increase in NF-kB, p50/p65 nuclear translocation, and cleavage of caspases 3 and 8, as well as transcriptional induction of caspase 12, Fas, IL-15, and the TNF receptor-associated ligand (TRAIL). It has been suggested that Poly(I:C) is one of the most appropriate generators of stable mature dendritic cells (DC). These mature DC might generate in vivo effective immune responses after injection due to their ability to secrete bioactive IL-12 after CD40 ligation. Poly (I:C) was used as a potent adjuvant to enhance the specific anti-tumor immune responses against a peptide-based vaccine.

포장

Package size based on polynucleotide content

기타 정보

Double-stranded homopolymer.

제조 메모

The product requires ionic strength to maintain the double-strand structure. Reconstitution at ~10 mg/mL of water yields a polynucleotide in physiological phosphate buffered solution.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)