R9010

Monoclonal Anti-Rat Kappa Light Chains (1a + 1b) antibody produced in mouse

clone RT-39, ascites fluid

• 동의어(들): Monoclonal Anti-Rat Kappa Light Chains (1a + 1b)
• MDL number: MFCD00164734
• UNSPSC 코드: 12352203
• NACRES: NA.46

속성

• 생물학적 소스: mouse
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: ascites fluid
• 항체 생산 유형: secondary antibodies
• 클론: RT-39, monoclonal
• 분자량: antigen 25 kDa
• 포함: 15 mM sodium azide
• 기술: capture ELISA: suitable; dot blot: suitable; immunocytochemistry: suitable; indirect ELISA: 1:2,000; western blot: suitable using denatured and reduced normal serum or myeloma protein
• 동형: IgG1
• 배송 상태: dry ice
• 저장 온도: −20°C
• 타겟 번역 후 변형: unmodified

설명

일반 설명

Monoclonal anti-Rat Light Chain (1a+1b) (mouse IgG1 isotype) is derived from the RT-39 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with purified rat IgG. An immunoglobulin (Ig) has two heavy chain and two light chain connected by disulfide bond. It is a glycoprotein. IgG is a major class of immunoglobulin. Rat has five immunoglobulin classes- IgM, IgG, IgA, IgD and IgGE. IgG has four subclasses- IgG1, IgG2a, IgG2b and IgG2c. Rat immunoglobulins have either κ or λ light chains. Greater than 90% of normal rat immunoglobulins and myelomas contain light chains.

특이성

The antibody reacts with an epitope on the rat κ light chain, which is shared by both the 1a and 1b allotypes in various rat immunoglobulin classes and subclasses. No cross-reactivity is observed with rat λ light chains or with IgG from human, mouse, bovine, rabbit, goat, sheep, horse, dog, chicken, pig or cat. The product shows weak cross-reaction with guinea pig IgG.

면역원

Purified rat IgG

애플리케이션

Monoclonal Anti-Rat κ Light Chains (1a + 1b) antibody has been used in capture enzyme linked immunosorbent assay (ELISA), immunoblotting, dot blot and immunocytochemistry.

Monoclonal Anti-Rat Kappa Light Chains (1a + 1b) antibody produced in mouse has been used in:

• enzyme-linked immunosorbent assay (ELISA)
• immunoblotting
• dot blot
• immunocytochemistry

생화학적/생리학적 작용

Immunoglobulin G (IgG) mainly helps in immune defense. It participates in hypersensitivity type II and type III reactions. It helps in opsonization, complement fixation and antibody dependent cell mediated cytotoxicity.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: nwg
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable