RDD008
Trizma® base
≥99.9%, powder, anhydrous, Redi-Dri™
동의어(들):
2-Amino-2-(hydroxymethyl)-1,3-propanediol, THAM, Tris base, Tris(hydroxymethyl)aminomethane, Trometamol
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모든 사진(6)
About This Item
Linear Formula:
NH2C(CH2OH)3
CAS Number:
Molecular Weight:
121.14
Beilstein:
741883
EC Number:
MDL number:
UNSPSC 코드:
12161700
PubChem Substance ID:
NACRES:
NA.25
추천 제품
Product Name
Trizma® base, anhydrous, free-flowing, Redi-Dri™, ≥99.9%
Grade
anhydrous
Quality Level
설명
aminopeptidase substrate
제품 라인
Redi-Dri™
분석
≥99.9%
양식
powder
품질
free-flowing
미포함
Carbon Fiber
불순물
≤0.2% Water (Karl Fischer)
색상
white
pH
10.5-12
7-9
유용한 pH 범위
7-9
pKa(25 °C)
8.1
bp
219-220 °C/10 mmHg (lit.)
mp
167-172 °C (lit.)
solubility
water: soluble (Water < 0.2%)
흡수
≤0.05 at 290 nm at 40%
λ
(A290 UV absorbance < 0.05)
in 5 mM Tris; DNA (40% (w/w))
SMILES string
NC(CO)(CO)CO
InChI
1S/C4H11NO3/c5-4(1-6,2-7)3-8/h6-8H,1-3,5H2
InChI key
LENZDBCJOHFCAS-UHFFFAOYSA-N
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일반 설명
Tris is an established basimetric standard and buffer used in biochemistry and molecular biology. It may be used by itself as a buffer or as a component of mixed buffer formulations, such as Tris-EDTA (TE) buffer, Tris-acetate-EDTA (TAE) buffer, Tris-borate-EDTA (TBE) buffer, etc. It is pure, essentially stable, relatively non-hygroscopic and has a high equivalent weight.
애플리케이션
Tris base has been used:
- To prepare Tris-HCl, which is used as a component of lysis buffer for protein characterization
- In the preparation of Tris-buffered saline Tween-20 (TBST) buffer for Western blotting
- In the preparation of TBS (Tris-buffered saline) that is used for dialysis to neutralize a protein
- As a component of nuclei extraction buffer
기타 정보
The pH values of all buffers are temperature- and concentration-dependent. For Tris buffers, pH increases about 0.03 unit per °C decrease in temperature, and decreases 0.03-0.05 unit per ten-fold dilution.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
For precise applications, use a carefully calibrated pH meter with a glass/calomel combination electrode.
법적 정보
Redi-Dri is a trademark of Sigma-Aldrich Co. LLC
Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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