S1797

SOC Medium

For use in transformation

• 동의어(들): Super Optimal broth
• UNSPSC 코드: 41106200
• NACRES: NA.85

속성

• Grade: Molecular Biology
• Quality Level: 500
• 무균: 0.2 μm filtered
• 양식: liquid
• 구성: Dextrose, 3.603 g/L ; KCl, 0.186 g/L ; MgSO₄, 4.8 g/L ; Tryptone, 20 g/L ; Yeast extract, 5 g/L
• 기술: microbiological culture: suitable
• 응용 분야: agriculture
• 저장 온도: 2-8°C
• 적합성: nonselective for Escherichia coli; nonselective for coliforms

설명

일반 설명

Super optimal medium with catabolic repressor (SOC) medium is a glucose-rich microbial growth medium used primarily in the recovery step of Escherichia coli competent cell transformations. The culture of E.coli cells in this nutrient-rich microbial broth increases the transformation efficiency of recombinant plasmids containing toxic protein genes by inhibiting the unintended activation of the lac promoter carried on the cloning vector. SOC medium contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation.

애플리케이션

SOC Medium has been used for the transformation of DH5α competent E-coli cells. It has also been used to culture white colonies post-cloning for the identification of the recombinant protein of interest.

특징 및 장점

SOC liquid provides:

• Filter-sterilized, ready-to-use format
• Convenient package sizes
• Standard formulation

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable