SAE0005

Glucose-6-phosphate Isomerase human

recombinant, expressed in E. coli

• 동의어(들): GPI, PGI
• EC 번호: 5.3.1.9 (BRENDA, IUBMB)
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 재조합: expressed in E. coli
• Quality Level: 200
• 분석: ≥95% (SDS-PAGE)
• 특이 활성도: ≥200 units/mg protein
• 저장 온도: −20°C

설명

생화학적/생리학적 작용

Glucose-6-phosphate isomerase (GPI) is a homodimeric enzyme ubiquitously present in most organisms. GPI catalyzes the interconversion between glucose-6-phosphate and fructose-6-hosphate, the second step of the glycolytic pathway. In mammals, Glucose-6-phosphate isomerase also acts as an autocrine motility factor (AMF), a neuroleukin, and a maturation factor. GPI deficiency is the second most common erythroenzymopathy of glycolytic enzymes after pyruvate kinase deficiency. Inherited deficiency of the enzymatic activity of GPI causes hereditary nonspherocytic hemolytic anemia (HNSHA) in humans, a severe deficiency that can be associated with hydrops fetalis (a-thalassaemia), immediate neonatal death, and neurological impairment.

물리적 특성

C-terminal histidine-tagged

단위 정의

One unit will convert 1.0 mmole of D-fructose 6-phosphate to D-glucose 6-phosphate per inute at pH 7.4 at 25 °C.

물리적 형태

Supplied as a solution in 50 mM Tris-HCl pH 7.5, and 50% glycerol

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable