SAE0097

D-2-Hydroxyglutarate Dehydrogenase (D2HGDH) from Acidaminococcus fermentans

recombinant, expressed in E. coli, aqueous solution

• 동의어(들): D2HGDH, HGDH, L-2-hydroxyglutarate dehydrogenase
• EC 번호: 1.1. 99.2
• UNSPSC 코드: 12352202
• NACRES: NA.54

속성

• 재조합: expressed in E. coli
• 분석: ≥95% (SDS-PAGE)
• 양식: aqueous solution
• 특이 활성도: ≥1000 units/mg protein
• UniProt 수납 번호: D2RJU7
• 배송 상태: wet ice
• 저장 온도: −20°C

설명

일반 설명

D-2-Hydroxyglutarate Dehydrogenase (D2HGDH) is a member of the D-2-hydroxyacid NAD⁺ dependent dehydrogenase family of proteins. D2HGDH catalyzes the conversion of α-ketoglutarate (α--KG) to D-2-hydroxyglutarate (D2HG), coupled to the oxidation of NADH to NAD⁺ .

The crystal structure of D2HGDH from Acidaminococcus fermentans has been reported. D2HGDH from Acidaminococcus fermentans has been used in several enzymatic assays, such as:

• A continuous spectrophotometric assay to measure the activity of aminotransferases, based on the transamination of a keto compound and L-glutamate, which yields a corresponding amino compound and 2-oxoglutarate.
• Determination of D2HG levels in biological fluids such as serum, urine, cell culture supernatants, and cell or tissue lysates.
• A coupled assay system to measure branched-chain amino acid aminotransferase activity.

단위 정의

One unit of enzyme oxidizes 1 μmole of NADH to NAD⁺ coupled to the reduction of α-ketoglutarate to (D)-2-hydroxyglutarate per minute at 37°C at pH 8.0.

제조 메모

This recombinant D2HGDH product is supplied as an aqueous solution in 20 mM Trizma^® buffer, pH 7.5, with 150 mM NaCl, and 10% glycerol.

법적 정보

Trizma is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable