SRP0255

eIF4E Active human

N-terminal GST-tag, recombinant, expressed in E. coli, ≥70% (SDS-PAGE)

• 동의어(들): CBP, Eukaryotic translation initiation factor 4E, mRNA cap-binding protein
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥70% (SDS-PAGE)
• 양식: aqueous solution
• 분자량: 51 kDa
• 포장: pkg of 100 μg
• 농도: >0.02 mg/mL
• 기술: cell based assay: suitable
• 적합성: suitable for molecular biology
• NCBI 수납 번호: NM_001968
• UniProt 수납 번호: P06730
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... EIF4E(1977)

설명

일반 설명

EIF4E (eukaryotic translation initiation factor 4E) is a m7GpppN (N is any nucleotide) cap-dependent RNA-binding protein. It is one of the three subunits of the translation initiation factor complex eIF4F, and is the least common of all initiation factors.

애플리케이션

Suitable substrate for MNK1 kinase.

생화학적/생리학적 작용

EIF4E (eukaryotic translation initiation factor 4E) is a part of eIF4F complex, which is responsible for the recruitment of ribosomes to the capped end of mRNAs during mRNA translation. It acts as the rate-limiting element in cap-dependent mRNA translation in eukaryotic cells. It promotes the translation of pro-oncogenic mRNAs, including vascular endothelial growth factor (VEGF), cyclin D3, and Mcl-1 (induced myeloid leukemia cell differentiation). Up-regulation of this protein is linked with various cancers, such as prostate cancer, breast cancer, and lymphoma. Studies show that this protein is overexpressed in melanoma cell lines and is linked with resistance to vemurafenib. Thus, it might have potential as a therapeutic target to suppress melanoma cell proliferation and abrogating vemurafenib resistance.

물리적 형태

Formulated in 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM DTT, 0.1 mM EGTA, 0.1 mM EDTA, 0.1 mM PMSF, 50% glycerol.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable