SRP0256

RB Active human

recombinant, expressed in E. coli, ≥80% (SDS-PAGE)

• 동의어(들): OSRC, RB1, Retinoblastoma 1, Retinoblastoma-associated protein, p105-Rb
• UNSPSC 코드: 12352200
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥80% (SDS-PAGE)
• 양식: aqueous solution
• 분자량: 43.5 kDa
• 포장: pkg of 200 μg
• 저장 조건: avoid repeated freeze/thaw cycles
• 농도: >0.02 mg/mL
• NCBI 수납 번호: NM_000321
• UniProt 수납 번호: P06400
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... RB1(5925)

설명

일반 설명

The gene encoding Retinoblastoma-associated protein (RB1) is a tumor suppressor gene localized on human chromosome 13q14.2. Human recombinant RB protein (773-end) with N-terminal GST tag (GenBank Accession No. NM_000321), expressed in a E. coli expression system.

애플리케이션

Retinoblastoma-associated protein (RB1) has been used in cell activity assay. It is also used as a kinase substrate for the kinase assay and in Western blotting.

생화학적/생리학적 작용

Retinoblastoma-associated protein (RB1) modulates the cell cycle. It acts as a repressor of transcription mediated by E2 factor (E2F) and influences transcriptional silencing. RB1 modulates mitochondrial activities and has a role in the organisation of heterochromatin. Mutations in the gene encoding this protein have been associated with childhood retinoblastoma.

물리적 형태

Formulated in 25 mM Tris-HCl, pH 8.0, 100 mM NaCl, 0.05% Tween-20, 30% glycerol and 3 mM DTT.

제조 메모

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable