SRP3234

SCF from mouse

Animal-component free, recombinant, expressed in E. coli, ≥98% (SDS-PAGE), ≥98% (HPLC), suitable for cell culture

• 동의어(들): Mast Cell Growth Factor (MGF), Steel Factor, Stem Cell Factor, c-Kit Ligand
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: mouse
• 재조합: expressed in E. coli
• 분석: ≥98% (HPLC); ≥98% (SDS-PAGE)
• 양식: lyophilized
• 효능: ≤10.0 ng/mL ED₅₀
• 분자량: 18.3 kDa
• 포장: pkg of 10 μg
• 기술: cell culture | mammalian: suitable
• 불순물: <0.1 EU/μg endotoxin, tested
• 색상: white
• UniProt 수납 번호: P20826
• 배송 상태: wet ice
• 저장 온도: −20°C
• 유전자 정보: mouse ... KITL(17311)

설명

일반 설명

Stem cell factor (SCF), which is also known as kit ligand (KITL), is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells. Recombinant murine SCF is an 18.3kDa polypeptide containing 164 amino acid residues, which corresponds to the sequence of the secreted soluble form of SCF.

생화학적/생리학적 작용

SCF and c-Kit are essential for the survival, proliferation and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. In mice, this protein has been shown to improve the development of ovarian follicles.

서열

MKEICGNPVT DNVKDITKLV ANLPNDYMIT LNYVAGMDVL PSHCWLRDMV IQLSLSLTTL LDKFSNISEG LSNYSIIDKL GKIVDDLVLC MEENAPKNIK ESPKRPETRS FTPEEFFSIF NRSIDAFKDF MVASDTSDCV LSSTLGPEKD SRVSVTKPFM LPPVA

물리적 형태

Lyophilized from 10 mM Sodium Phosphate, pH 6.5.

재구성

Centrifuge the vial prior to opening. Reconstitute in water to a concentration of 0.1-1.0 mg/ml. Do not vortex. This solution can be stored at 2-8°C for up to 1 week. For extended storage, it is recommended to further dilute in a buffer containing a stabilizer (example 5% Trehalose) and store in working aliquots at -20°C to -80°C.

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable