SRP5160

14-3-3 σ, untagged human

recombinant, expressed in E. coli, ≥70% (SDS-PAGE), buffered aqueous glycerol solution

• 동의어(들): 14-3-3 σ, SFN, YWHAS, stratifin
• CAS Number: 1173938-21-0
• UNSPSC 코드: 12352202
• NACRES: NA.32

속성

• 생물학적 소스: human
• 재조합: expressed in E. coli
• 분석: ≥70% (SDS-PAGE)
• 양식: buffered aqueous glycerol solution
• 분자량: ~29 kDa
• NCBI 수납 번호: NM_006142
• 응용 분야: cell analysis
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... SFN(2810)

설명

일반 설명

14-3-3σ or stratifin is a protein that is strongly induced by gamma irradiation and other DNA-damaging agents. The induction of 14-3-3σ is mediated by a p53 responsive element. Exogenous introduction of 14-3-3σ into cycling cells results in a G2 cell cycle arrest. Knockout of 14-3-3σ in cells showed that the cells are unable to maintain cell cycle arrest after DNA damage. The 14-3-3σ -/- cells die as they enter mitosis. This process is associated with a failure of the 14-3-3σ-deficient cells to sequester the proteins that initiate mitosis and prevent them from entering the nucleus. Thus, 14-3-3σ plays an important role in maintaining the G2 checkpoint in cells and preventing mitotic death.

물리적 형태

Supplied in 50mM Tris-HCl, pH 7.5, 50mM NaCl, 0.25mM DTT, 0.1mM PMSF, 25% glycerol.

제조 메모

after opening, aliquot into smaller quantities and store at -70 °C. Avoid repeating handling and multiple freeze/thaw cycles

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable