T6763

Trypsin Acetylated from bovine pancreas

Type V-S, ≥8,500 BAEE units/mg protein (biuret)

• 동의어(들): Acetyltrypsin
• EC 번호: 3.4.21.4 (BRENDA, IUBMB)
• MDL number: MFCD00082094
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 생물학적 소스: bovine pancreas
• Quality Level: 200
• 유형: Type V-S
• 양식: powder
• 특이 활성도: ≥8,500 BAEE units/mg protein (biuret)
• UniProt 수납 번호: Q29463
• 저장 온도: −20°C
• 유전자 정보: cow ... TRYP8(282603)

설명

일반 설명

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

애플리케이션

Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter.

생화학적/생리학적 작용

Lys-acetylated trypsin is less susceptible to autolysis than the native form.

단위 정의

One BAEE unit will produce a ΔA₂₅₃ of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

제조 메모

Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).

기타 정보

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer.

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H334,H335
• 예방조치 성명서: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Faceshields, Gloves