T7513

Thrombin from bovine plasma

lyophilized powder, ≥2,000 NIH units/mg protein (E1%/280 = 19.5)

• 동의어(들): Factor IIa
• CAS Number: 9002-04-4
• EC 번호: 3.4.21.5 (BRENDA, IUBMB)
• EC Number: 232-648-7
• MDL number: MFCD00166053
• UNSPSC 코드: 12352204
• NACRES: NA.54

속성

• 양식: lyophilized powder
• Quality Level: 200
• 특이 활성도: ≥2,000 NIH units/mg protein (E1%/280 = 19.5)
• 분자량: heavy chain ~33 kDa; light chain ~5 kDa
• UniProt 수납 번호: P00735
• 응용 분야: diagnostic assay manufacturing
• 저장 온도: −20°C
• 유전자 정보: cow ... F2(280685)

설명

생화학적/생리학적 작용

Serine protease that selectively cleaves Arg-Gly bonds in fibrinogen to form fibrin and fibrinopeptides A and B.

단위 정의

Activity is expressed in NIH units obtained by direct comparison to a NIH thrombin reference standard.

물리적 형태

Lyophilized from saline sodium citrate buffer, pH 6.5

분석 메모

Activity is expressed in NIH units obtained by direct comparison to a NIH Thrombin Reference Standard, Lot K.

The NIH assay procedure uses 0.2 mL of diluted plasma (1:1 with saline) as a substrate and 0.1 mL of thrombin sample (stabilized in a 1% buffered albumin solution) based on a modification of the method of Biggs. Only clotting times in the range of 15-25 seconds are used for determining thrombin concentrations.

기타 정보

View more information on thrombin at www.sigma-aldrich.com/enzymeexplorer.

안전성 정보

• 픽토그램: GHS08,GHS07
• 신호어: Danger
• 유해 및 위험 성명서: H315,H319,H334,H335
• 예방조치 성명서: P261 - P264 - P271 - P280 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable