U5382

Ubiquitin human

≥95% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal FLAG^® tagged), lyophilized powder

• CAS Number: 60267-61-0
• MDL number: MFCD02100852
• UNSPSC 코드: 41106500
• NACRES: NA.32

속성

• 생물학적 소스: human
• Quality Level: 200
• 재조합: expressed in E. coli (N-terminal FLAG^® tagged)
• 분석: ≥95% (SDS-PAGE)
• 양식: lyophilized powder
• 분자량: 10 kDa
• 기술: mass spectrometry (MS): suitable
• solubility: 0.05 M Tris pH 7.5: ≥10 mg/mL, clear to slightly hazy, colorless; 0.05 M Tris pH 7.5: ≥10 mg/mL
• UniProt 수납 번호: P0CG47; P0CG48
• 저장 온도: −20°C
• 유전자 정보: human ... UBB(7314), UBC(7316)

설명

일반 설명

Research Area: CANCER
Ubiquitin is a highly conserved protein composed of 76 amino acids, and it is expressed universally in all eukaryotes, ranging from yeast to humans.

Ubiquitin is a highly conserved globular protein and has lysine in the surface. The C-terminal end comprises the Leu-Arg-Gly-Gly structural motif.

애플리케이션

Ubiquitin human can be used as a standard for mass spectrometry.

Ubiquitin human has been used in mono-ubiquitination of yeast proliferating cell nuclear antigen (PCNA) and in in vitro ubiquitination assay of defective in mitotic arrest 1 (Dma1)

Ubiquitin, N-terminal FLAG-tagged can replace the ubiquitin in formation of poly-ubiquitin—protein conjugates. The FLAG tag enables separation and enrichment of the protein conjugates on anti-FLAG affinity columns and detection of conjugates in western blot by anti-FLAG antibodies.

생화학적/생리학적 작용

Ubiquitin interacts with the lysine residue of proteins through its ε-amino group of the C-terminal glycine residue. Proteins interacting with ubiquitin either undergo mono-ubiquitination or multi-mono-ubiquitination via a three-step process. Ubiquitination regulates intracellular trafficking and protein degradation and an imbalance in the pathway is implicated in disorders.

Ubiquitin is a small regulatory protein present in eukaryote tissues. Exogenous ubiquitin can stimulate apoptosis in numerous cell lines. E7 protein of human papilloma virus-16 stimulates Retinoblastoma Protein degradation via Ubiquitin-Proteasome Pathway.

Ubiquitin-mediated proteolysis plays an important role in several basic cellular processes including regulation of cell cycle and division, differentiation and development, modulation of cell-surface receptors, the secretory pathway (protein transport), morphogenesis of neuronal networks, transcriptional regulation and signal transduction, transcriptional silencing, DNA repair, long-term memory, and circadian rhythms.

포장

Package size based on protein content

제조 메모

Ubiquitin human can be dissolved in 0.05 M Tris-HCl at a concentration of 10.00 - 11.00 mg/ml to yield a clear to slightly hazy, colorless solution.

법적 정보

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

안전성 정보

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, type N95 (US)