V1514

Anti-VE-Cadherin (CD144) antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

• 동의어(들): Anti-CD144, Anti-Cadherin-5, Anti-Vascular Endothelial Cadherin
• MDL number: MFCD01633075
• UNSPSC 코드: 12352203
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 200
• 결합: unconjugated
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 양식: buffered aqueous solution
• 분자량: antigen ~130 kDa
• 종 반응성: human
• 기술: immunohistochemistry (frozen sections): 1:100 using acetone-fixed sections of human tonsil; western blot: 1:500 using whole extract of human umbilical vein endothelial cells (HUVEC).

설명

일반 설명

Cadherin 5 (CDH5), also known as cadherin 144 (CD144), is encoded by the gene mapped to long arm of human chromosome 16. The encoded protein is expressed in endothelial cells, but it absent in normal epithelium.

VE-Cadherin (also designated vascular endothelial cadherin, cadherin-5, and cluster of differentiation 144 (CD144)) is a non-type I or II cadherin that is expressed on endothelial cells of all blood and lymphatic vessels, where it organizes intercellular adherens junctions.

면역원

synthetic peptide corresponding to amino acids 91-110 of human VE-cadherin (CD144).

애플리케이션

Anti-VE-Cadherin (CD144) antibody produced in rabbit has been in following studies:

• immunofluorescence imaging[1]
• western blotting[2]
• to weaken cell-cell junctions in human umbilical vein endothelial cells (HUVECs)
• immunohistochemistry

생화학적/생리학적 작용

Cadherin 5 (CDH5)/CD144 plays an essential role in various cellular processes, such as regulation of cell adhesion, cell proliferation, cell survival, cell shape, cell motility. Additionally, it is also involved in the regulation of signaling pathways and transcriptional gene regulation. CD144/VE-cadherin is a core component of endothelial adherens junction, which plays a vital role in angiogenic sprouting by enhancing cell migration and cell elongation. CD144 expressed in glioblastoma multiforme (GBM) facilitates the transdifferentiation of glioblastoma stem like cells (GSCs) to endothelial cells and form blood vessels, under hypoxia condition. VE-cadherin induces endothelial cell-cell adhesion at adherens junctions (AJs). CD144 gene knockout results in loss of cell polarity and modification in vascular lumenal structure. CD144 inhibits the synthesis of CDH2 (also known as N-cadherin) in the endothelium.

VE-cadherin (CD144) interacts with β-catenin, plakoglobinand Adherens junction protein (p120ctn).

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable