V4014

Anti-V5-Cy3^™ antibody, Mouse monoclonal

purified immunoglobulin, ~1 mg/mL, clone V5-10

• 동의어(들): Monoclonal Anti-V5
• MDL number: MFCD03457579
• UNSPSC 코드: 12352203
• NACRES: NA.56

속성

• 생물학적 소스: mouse
• 결합: CY3 conjugate
• 항체 형태: purified immunoglobulin
• 항체 생산 유형: primary antibodies
• 클론: V5-10, monoclonal
• 농도: ~1 mg/mL
• 기술: immunocytochemistry: 2.0-4.0 μg/mL
• 배송 상태: wet ice
• 저장 온도: 2-8°C

설명

일반 설명

Monoclonal Anti-V5 (mouse IgG1 isotype) is derived from the V5 hybridoma, produced by fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with a synthetic peptide corresponding to amino acid residues GKPIPNPLLGLDST (95-108) of the P/V proteins of the Paramyxovirus SV5, conjugated to KLH. The conjugate is then extensively dialyzed to remove unbound Cy3 fluorophore. Monoclonal Anti-V5, Cy3 conjugate recognizes the V5 tag sequence on V5 tagged fusion proteins by immunocytochemistry.

특이성

Recognizes V5 Tag (GKPIPNPLLGLDST) fusion proteins

애플리케이션

Many recombinant proteins have been engineered to express a short sequence derived from the V5 molecule known as V5-tag. This tag facilitates the detection, isolation and purification of the proteins. Monoclonal antibodies reacting specifically with V5 and conjugated to Cy3 may be useful in various immunotechniques, and also to identify the expression of a V5 tagged fusion protein in situ by immunofluorescence.

물리적 형태

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide.

법적 정보

Cy3 is a trademark of Cytiva

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable