추천 제품
생물학적 소스
rabbit (recombinant)
Quality Level
재조합
expressed in HEK 293 cells
결합
unconjugated
항체 형태
purified antibody
항체 생산 유형
primary antibodies
클론
3B18, recombinant monoclonal
monoclonal
제품 라인
ZooMAb® learn more
양식
lyophilized
분자량
calculated mol wt 29.16 kDa
observed mol wt ~30 kDa
종 반응성
rat, monkey, human
포장
antibody small pack of 25 μL
환경친화적 대안 제품 특성
Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.
향상된 검증
recombinant expression
Learn more about Antibody Enhanced Validation
sustainability
Greener Alternative Product
기술
affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
western blot: suitable
동형
IgG
UniProt 수납 번호
환경친화적 대안 카테고리
, Aligned
배송 상태
ambient
저장 온도
2-8°C
타겟 번역 후 변형
unmodified
유전자 정보
human ... LHPP(64077)
일반 설명
We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.
Learn more about ZooMAb here.
특이성
Clone 3B18 is a ZooMAb rabbit recombinant monoclonal antibody that specifically detects Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP).
면역원
His-tagged full-length recombinant human Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (LHPP).
애플리케이션
Anti-LHPP, clone 3B18 ZooMAb, Cat. No. ZRB1050, is a recombinant Rabbit monoclonal antibody that specifically targets LHHP and is tested for use in Affinity Binding Assay, Immunocytochemistry, Immunohistochemistry (Paraffin), and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected LHPP in lysates from rat kidney, human liver, and COS-7 cells.
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LHPP in A431 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected LHPP in human liver tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound human recombinant LHPP with a KD of 8.6 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected LHPP in A431 cells.
Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected LHPP in human liver tissue sections.
Affinity Binding Assay: A representative lot of this antibody bound human recombinant LHPP with a KD of 8.6 x 10-7 in an affinity binding assay.
Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user
표적 설명
Phospholysine phosphohistidine inorganic pyrophosphate phosphatase (UniProt: Q9H008; also known as EC:3.6.1.1, Hlhpp, LHPP) is encoded by the LHPP gene (Gene ID: 64077) in human. LHPP is an evolutionarily conserved member of the HAD-like hydrolase superfamily with phosphatase activity. It displays broad substrate specificity and can hydrolyze imidodiphosphate, 3-phosphohistidine, and 6-phospholysine. It can also hydrolyze inorganic diphosphate, but with reduced efficiency. It has two substrate binding sites that are located in amino acids 17-19 and 54-55. Its higher expression has been reported in brain, and somewhat lower expression in liver and kidney. LHHP expression is shown to be down regulated in liver and bladder cancers and knockdown of LHPP promotes the proliferation and growth of tumor cells. Conversely, sustained hepatic expression of LHPP in a murine model of hepatocellular carcinoma is reported to reduce tumor burden and prevent the loss of liver function. Silencing of LHHP can lead to enhanced phosphorylation of Akt and p65 and up-regulates the level of Bcl-2 and cyclin D1. Two isoforms of LHPP have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Hindupur, SK., et al. (2018). Nature. 555(7698); 678-682; Li, Y., et al. (2019). Biosci Rep. 39(7); BSR20182270.).
물리적 형태
Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.
재구성
Reconstitute lyophilized antibody pellet with 25μL of ultrapure water or Phosphate Buffered Saline (PBS). Please refer to our reconstitution protocol and the specific application guidance on the suggested starting dilutions and sample type.
저장 및 안정성
Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.
법적 정보
ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
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