567517-U

Discovery^® HS F5 (5 µm) HPLC Columns

L × I.D. 25 cm × 4.6 mm, HPLC Column

• 동의어(들): Discovery Pentafluorophenyl PFP HPLC Column
• UNSPSC 코드: 41115700
• eCl@ss: 32110501
• NACRES: SB.52

속성

• Product Name: Discovery^® HS F5 HPLC Column, 5 μm particle size, L × I.D. 25 cm × 4.6 mm
• material: stainless steel column
• Quality Level: 100
• Agency: suitable for USP L43
• 제품 라인: Discovery^®
• 특징: endcapped
• 제조업체/상표: Discovery^®
• 포장: 1 ea of
• 라벨링 범위: 12% Carbon loading
• 파라미터: ≤70 °C temp. range; 400 bar pressure (5801 psi)
• 기술: HPLC: suitable; LC/MS: suitable
• 길이 × I.D.: 25 cm × 4.6 mm
• 표면적: 300 m²/g
• 표면 범위: 4 μmol/m²
• 불순물: <10 ppm metals
• Matrix: silica gel, high purity, spherical particle platform; fully porous particle
• 기질 활성군: PFP (pentafluorophenyl) phase
• 입자 크기: 5 μm
• 공극 크기: 120 Å
• 작동 pH 범위: 2-8
• 응용 분야: food and beverages
• 분리 기술: reversed phase

설명

일반 설명

Guidelines for transferring a C18 method to Discovery® HS F5:
Generally, bases are retained longer on the HS F5 than on a C18. Increasing the organic content of a C18 separation 5 to 10 percent will generally provide similar retention on an HS F5. Results with other compounds are highly variable. However, it is generally true that solutes with log P_o/w values less than 2.5 will be retained longer on HS F5 compared to a C18. The degree of difference is highly solute dependent.

The Discovery® HS F5 bonded phase provides reversed-phase separations that are distinctly different from C18 columns. However, compounds will generally elute within the same retention time window, making most C18 methods easily transferable.

애플리케이션

• Determination of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline, 5-hydroxytryptamine and 5-hydroxyindole acetic acid in the neonatal rat brains using high performance liquid chromatography-electrochemical detection.: This study highlights the use of the Discovery^® HS F5 HPLC Column for the sensitive detection of neuroactive compounds in neonatal rat brain samples, advancing our understanding of neurological development and neurotransmitter dynamics (Mao et al., 2009).


• Advantages of pentafluorophenylpropyl stationary phase over conventional C18 stationary phase--application to analysis of triamcinolone acetonide.: This research showcases the superior performance of the Discovery^® HS F5 HPLC Column in analyzing corticosteroid formulations, providing insights that could lead to improved analytical methodologies in pharmaceutical analysis (Havlikova et al., 2008).


• Determination of ephedrine alkaloids in Ephedra natural products using HPLC on a pentafluorophenylpropyl stationary phase.: Utilizes the Discovery^® HS F5 HPLC Column for the precise and efficient separation of ephedrine alkaloids, contributing to safer and more effective use of Ephedra in herbal supplements (Pellati and Benvenuti, 2008).


• Fast high-performance liquid chromatography analysis of phenethylamine alkaloids in Citrus natural products on a pentafluorophenylpropyl stationary phase.: Demonstrates the efficiency of the Discovery^® HS F5 HPLC Column in the rapid analysis of phenethylamine alkaloids, enhancing the quality control of Citrus-based dietary supplements (Pellati and Benvenuti, 2007).


• High-performance liquid chromatographic method with UV photodiode-array, fluorescence and mass spectrometric detection for simultaneous determination of galantamine and its phase I metabolites in biological samples.: This study uses the Discovery^® HS F5 HPLC Column to simultaneously quantify galantamine and its metabolites, aiding in the pharmacokinetic profiling of this Alzheimer′s disease medication (Malakova et al., 2007).

특징 및 장점

• Unique selectivity
• Similar retention to C18 (sometimes requires stronger mobile phase)
• Excellent peak shape
• Stable, low-bleed LC-MS separations
• Scalable separations from 3 to 10μm particle sizes

추천 제품

Discover LiChropur reagents ideal for HPLC or LC-MS analysis

법적 정보

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany