기술
HPLC: suitable
검사 파라미터
column: BIOshell A160 Peptide C18, 15 cm x 2.1 mm I.D., 2.7 μm particles (66905-U)
mobile phase: [A] 75:25, 0.1% TFA in water: 0.08% TFA in acetonitrile; [B] 25:75, 0.1% TFA in water: 0.08% TFA in acetonitrile
gradient: held at 0% B for 0.5 min; 0 to 100% B in 7 min
flow rate: 0.3 mL/min
pressure: 4743 psi (327 bar)
column temp.: 35 °C
detector: UV, 220 nm
injection: 1 μL
sample: 0.2 - 0.6 g/L in mobile phase A
적합성
application for HPLC
응용 분야
life science and biopharma
법적 정보
분석물
설명
Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein
Adrenomedullin rat, ≥95% (HPLC)
Cytochrome c from chicken heart, ≥95% based on Mol. Wt. 12,222 basis
Lysozyme from chicken egg white, powder or granules, ≥39,000 units/mg protein
Superoxide Dismutase from bovine erythrocytes, ≥3,000 units/mg protein, BioReagent, lyophilized powder, suitable for cell culture
Trypsin Inhibitor from Glycine max (soybean), BioUltra, lyophilized powder, ≥95% (Kunitz inhibitor, SDS-PAGE)
Carbonic Anhydrase from bovine erythrocytes, lyophilized powder, ≥2,000 W-A units/mg protein
Albumin from chicken egg white, lyophilized powder, ≥98% (agarose gel electrophoresis)
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