33247

Urea

puriss. p.a., ACS reagent, reag. Ph. Eur., ≥99%

• Synonym(s): Carbamide, Carbonyldiamide
• Linear Formula: NH₂CONH₂
• CAS Number: 57-13-6
• Molecular Weight: 60.06
• Beilstein: 635724
• EC Number: 200-315-5
• MDL number: MFCD00008022
• UNSPSC Code: 12352100
• PubChem Substance ID: 57648752
• NACRES: NA.21

Properties

• grade: ACS reagent; puriss. p.a.
• Quality Level: 200
• Agency: reag. Ph. Eur.
• Assay: ≥99%; 99-101% (calc. on dry substance)
• form: solid
• impurities: alkalic reac. substances, complies; ≤0.0005% heavy metals (as Pb); ≤0.05% ammonium (NH₄); ≤0.1% biuret
• ign. residue: ≤0.01% (as SO₄)
• loss: ≤1.0% loss on drying, 105°C, 1 h
• mp: 132-135 °C (lit.); 132-135 °C
• solubility: H₂O: soluble 480 g/L at 20 °C
• density: 1.335 g/mL at 25 °C (lit.)
• anion traces: chloride (Cl^-): ≤5 mg/kg; sulfate (SO₄^2-): ≤10 mg/kg
• cation traces: Fe: ≤2 mg/kg
• suitability: in accordance for appearance of solution; in accordance for identity
• functional group: amine
• SMILES string: NC(N)=O
• InChI: 1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)
• InChI key: XSQUKJJJFZCRTK-UHFFFAOYSA-N
• Gene Information: human ... CA1(759), CA2(760) ; rat ... Ppm1a(24666)

Description

General description

Urea is a colorless, odorless solid. It has prism-like crystals. It forms insoluble nitrate salts with nitric acid and oxalate salts with oxalic acid. On reaction with alkanes or substituted alkanes, it affords crystal-lattice inclusion compounds.

Urea is the most commonly used slow release nitrogen fertilizer.

Application

Urea has been used in the following studies:

• Synthesis of SnO₂ nanoparticles.
• Synthesis of urethanes, ureides and semicarbazides.
• Denaturation of protein isolated from Saccharomyces cerevisiae Y15696 strain.
• Protein electrophoretic studies.

Used for the denaturation of proteins and as a mild solubilization agent for insoluble or denatured proteins. Useful for renaturing proteins from samples already denatured with 6 M guanidine chloride such as inclusion bodies. May be used with guanidine hydrochloride and dithiothreitrol (DTT) in the refolding of denatured proteins into their native or active form.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves